Antagomir nc

MiR-455-4p mimic AgomiR-455-5p, agomir-NC, MiR-455-4p antagonist antagomiR-455-5p, and antagomir-NC (all provided by Sangon Biotech, Shanghai, China) were dissolved at a concentration of 0.08 nmol in 4 μL of 0.01 M phosphate buffered saline (PBS) and were administrated via intracerebroventricular injection 2 hours before or 0 and 1 hour after the MCAO (Zuo et al., 2019) (Additional Figure 1 for the experimental design). Mice were fixed in a stereotactic head frame (RWD Life Science). The head skin was cut along the midline and a burr hole was bored in the left side of the skull (1.0 mm lateral and 0.5 mm posterior to the bregma). Using a Hamilton syringe and a microinfusion pump (KDS 310, KD Scientific, Holliston, MA, USA), agomiR-455-5p, agomir-NC, antagomiR-455-5p, or antagomir-NC was infused into the left lateral ventricle at 0.2 μL/min. After injection, the needle was left for an additional 5 minutes to prevent possible leakage, then withdrawn slowly. The burr hole was sealed with bone wax and the incision was closed.