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Infusion syringe pump

Manufactured by Stoelting

The Infusion Syringe Pump is a laboratory equipment designed to precisely deliver controlled volumes of liquid or solution at a consistent flow rate. It is used to infuse medications, solutions, or other fluids into a patient or experimental subject. The pump utilizes a motor-driven mechanism to push a syringe plunger, allowing for accurate and reliable fluid delivery.

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2 protocols using infusion syringe pump

1

Microdialysis in Awake Mouse Hippocampus

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Microdialysis was performed in the hippocampus of awake and normally behaving mice housed in a “Raturn” cage system (Bioanalytical Systems) as previously described (Cirrito et al., 2003 (link); Hong et al., 2011 (link)). 1.5% bovine serum albumin in artificial CSF (in mM: 1.3 CaCl2, 1.2 MgSO4, 3 KCl, 0.4 KH2PO4, 25 NaHCO3, and 122 NaCl, pH 7.35) was perfused using BR-2 probes with 35-kDa MWCO membranes (Bioanalytical Systems) at 0.2 or 0.4 μl/min using an infusion syringe pump (Stoelting).
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2

Intracerebral Microdialysis in Mice

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These mice had an intracerebral guide cannula implanted using coordinates for right hippocampal dentate gyrus placement (bregma−1.9 mm, midline−1.1 mm, and dura−1.5 mm). After inserting probes with 6 kDa molecular weight cutoff (MWCO) membranes (CMA), artificial CSF (in mM: 1.3 CaCl2, 1.2 MgSO4, 3 KCl, 0.4 KH2PO4, 25 NaHCO3, and 122 NaCl, pH 7.35, filtered and degassed) was perfused at flow rates 1.0 μl/min with an infusion syringe pump (Stoelting). Microdialysates were collected in a fraction collector at 4°C every hour. Mice were housed in a Raturn cage system (Bioanalytical Systems), which allowed normal movements and activity. All ISF samples were snap‐frozen and stored at −80°C until further analysis.
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