Eos rebel t3i

To visualize the penetration of applied substances into the leaf, a fluorescent analog of glucose solution (NBDG: 2-NBDG = 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose. λ Ex/Em (nm) 465/540) was applied to the leaf surface. The NBDG solution was prepared and used at a concentration of 30 mM.
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Microscopic observations were performed using a Carl Zeiss™ Axio Scope A-1
^® microscopy platform equipped with a Canon™ EOS Rebel T3i
^® camera and a Carl Zeiss™ AxioCam ICc 1
^®. Low magnification images were obtained using a Zeiss™ Stemi SV11
^® fluorescent stereoscope (Carl Zeiss Microscopy™+ GmbH, Göttingen, Germany).

Microscopic observations were made using a Carl Zeiss Axion Scope A-1 equipped with a Canon EOS Rebel T3i camera and a Carl Zeiss AxioCam ICc1. Low magnification images were taken with a Zeiss Stemi SV11 fluorescent stereoscope (Carl Zeiss Microscopy, Oberkochen, Germany).