Merck sequant zic hilic column

MS measurements were performed in randomized order in three batches (serum, urine Ut1, urine Ut2) on a Thermo Fischer Ultimate 3000 UHPLC system (Thermo Fischer Scientific, San Jose, CA, USA) coupled to a HR TOF instrument system (TripleTOF 6600, Sciex, Concord, ON, Canada) as described elsewhere and in the supplementary information [13 (link),23 (link),24 (link)]. Briefly, two different columns—RP (Waters XSelect HSST RP-C18 column, Waters, Baden-Daettwil, Switzerland) (150 mm × 2.1 mm, 2.5 µm particle size)) and HILIC (Merck SeQuant ZIC HILIC column, Merck, Darmstadt, Germany) (150 mm × 2.1 mm, 3.5 µm particle size)) were used for chromatographic separation. HR MS and MS/MS data were acquired by two methods: TOF MS only and information dependent data acquisition (IDA) separated in positive and negative ionization mode (resolving power 30,000 in MS and 15,000 in MS/MS). A SST described in detail in reference [24 (link)] was measured after every fifth sample and was checked for reproducibility of the data by retention time (RT) shifts and peak area comparison using MultiQuant V 2.1 (Sciex, Concord, ON, Canada). Further, a pooled QC sample was additionally measured after every fifth sample.