Bca protein average concentration detection kit

Nicotinic acid riboside (concentration ≥98%, Maclin, Shanghai, China, batch number: C12093435), doxorubicin (concentration≥99.5%, batch number: HBW200804-3, Hubei Weideli Co., Ltd.), dexrazoxane (concentration ≥99.5%, batch number: HBW200701-3, Hubei Weideli Chemical Technology Co., Ltd.), BCA protein average/concentration detection kit (purchased from Dalian Meilun Biotechnology Co., Ltd.), ATP detection kit, mitochondrial membrane matrix detection kit, TUNEL kit, NAD⁺/NADH detection kit, GSH detection kit, SOD activity detection kit, and lipid oxidation MDA detection kit (all detection kits are purchased from Beyotime Biotechnology Company). All other chemicals and reagents were of analytical grade. DMEM, fetal bovine serum, and cell lysis buffer (10×) were from Meilun Biotech (Dalian, China). Nrf-2, Keap-1, RhoA, ROCK-1, HRP-conjugated GAPDH, LC3B, p62, Beclin 1, Bcl-2, caspase-3, Bax, and IL-4 (antibodies were purchased from ABclonal).

ISL, EZ (purity > 95%), and (R)-Mevalonic acid lithium salt were purchased from Sigma, New York, NY, USA. Niemann-Pick C1-like 1, β-actin, β-tubulin (antibodies were purchased from ABclonal, Wuhan, China), fetal bovine serum, Dulbecco’s modified Eagle’s medium (DMEM) and cell lysis buffer (10×), secondary antibody horseradish peroxidase-conjugated anti-rabbit (L3012), ultra-enhanced chemiluminescence detection reagent, Filipin bacteriocin, and BCA protein average/concentration detection kit were all purchased from Meilun Biotechnology (Dalian, China). Sodium taurocholate hydrate and lovastatin were purchased from Maclean’s, NBD-labeled cholesterol (from J&K scientific, Beijing, China).