Hci image capture software

Manufactured by Hamamatsu Photonics

HCI image capture software is a tool developed by Hamamatsu Photonics for acquiring and processing digital images. The software provides basic functionalities for image capture, display, and basic analysis.

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Lab products found in correlation

Bx61wi microscope, by Olympus (2 mentions) Flynap, by Carolina Biological Supply (2 mentions) Orca flash4 cmos digital camera, by Hamamatsu Photonics (1 mentions) Bx61wi microscope, by Hamamatsu Photonics (1 mentions)

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HCI image software (3 citations)

4 protocols using hci image capture software

Assessing Drosophila Heart Function via SOHA

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Assessment of Drosophila heart function and structure using the Semi-automatic Optical Heartbeat Analysis (SOHA) method as previously described [74 (link), 75 (link)]. Briefly, four-day old adult flies were anaesthetized with FlyNap (Carolina Biological Supply Co, Burlington, NC) and dissected in oxygenated artificial hemolymph to expose the beating heart within the abdomen. Hearts were placed on an Olympus BX61WI microscope while being filmed through a 10x water immersion lens with a high-speed digital camera (Hamamatsu Photonics C9300 digital camera) using HCI image capture software (Hamamatsu). High-speed movies were analyzed using the Semi-automated Optical Heartbeat Analysis (SOHA) software [74 (link), 75 (link)]. Parameters measured include Heart Period (HP), Diastolic Interval (DI), Systolic Interval (SI), Arrhythmicity Index (AI), Diastolic Diameter (DD), Systolic Diameter (SD) and Fractional Shortening (FS) (Fig 2A). FS, a measure of contractility, is calculated using the following equation FS = (DD-SD)/DD.

Schroeder A.M., Nielsen T., Lynott M., Vogler G., Colas A.R, & Bodmer R. (2022). Nascent polypeptide-Associated Complex and Signal Recognition Particle have cardiac-specific roles in heart development and remodeling. PLOS Genetics, 18(10), e1010448.

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Fly Heart Contractility Analysis

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Analysis of cardiac contractile parameters was performed on surgically
exposed fly hearts using high-resolution video microscopy, as previously
described (Fink et al., 2009 (link)). Briefly,
flies were dissected to expose the heart within the abdomen and were kept in
oxygenated artificial hemolymph. Sub-merged dissected hearts were oxygenated for
15 min at room temperature to equilibrate. High-speed digital movies were made
with a Leica DM-LFSA microscope with a 10 3 water immersion lens, a Hamamatsu
EM-CCD camera, and HCI image capture software (Hamamatsu). High-speed digital
movies were recorded and analyzed using the semiautomatic optical heartbeat
analysis (SOHA) software developed in-house. Fractional shortening (FS),
commonly used by cardiologists as an important parameter of contractility, is
calculated from diastolic diameter (DD) and SD (FS = DD-DS/DD).

Walls S.M., Cammarato A., Chatfield D.A., Ocorr K., Harris G.L, & Bodmer R. (2018). Ceramide-Protein Interactions Modulate Ceramide-Associated Lipotoxic Cardiomyopathy. Cell reports, 22(10), 2702-2715.

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Drosophila Heart Dissection and Analysis

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All dissection steps were done using artificial hemolymph. In brief, 3-week-old female flies were anesthetized with FlyNap (Carolina Biological), transferred to a petroleum jelly-coated Petri dish, and dissected as described (Vogler and Ocorr 2009 ). The dissected hearts were equilibrated for 15 min at room temperature under constant oxygenation. High-speed movies were taken on an Olympus BX61WI microscope with a 10x immersion objective, using a Hamamatsu Orca Flash4 CMOS digital camera and HCI image capture software (Hamamatsu). Movies were then analyzed with custom-designed software (Ocorr et al. 2009 ).

Sloutskin A., Itzhak D., Vogler G., Ideses D., Alter H., Shachar H., Doniger T., Frasch M., Bodmer R., Duttke S.H, & Juven-Gershon T. (2023). A single DPE core promoter motif contributes to in vivo transcriptional regulation and affects cardiac function. bioRxiv.

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Drosophila Heart Functional Analysis

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Assessment of Drosophila heart function and structure using the Semi-automatic Optical Heartbeat Analysis (SOHA) method as previously described [77, 78] . Briefly, four-day old adult flies were anaesthetized with FlyNap (Carolina Biological Supply Co, Burlington, NC) and dissected in oxygenated artificial hemolymph to expose the beating heart within the abdomen.
Hearts were placed on an Olympus BX61WI microscope while being filmed through a 10x water immersion lens with a high-speed digital camera (Hamamatsu Photonics C9300 digital camera) using HCI image capture software (Hamamatsu). High-speed movies were analyzed using the Semi-automated Optical Heartbeat Analysis (SOHA) software [77, 78] . Parameters measured include Heart Period (HP), Diastolic Interval (DI), Systolic Interval (SI), Arrhythmicity Index (AI), Diastolic Diameter (DD), Systolic Diameter (SD) and Fractional Shortening (FS) (Figure 2A). FS, a measure of contractility, is calculated using the following equation FS=(DD-SD)/DD.

Schroeder A.M., Vogler G., Colas A.R., & Bodmer R. (2022). Nascent Polypeptide Associated Complex–alphaand Signal Recognition Particle are required for cardiac development and remodeling.

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