For mouse Abs: isotype control Abs (IgG1, IgG2a or IgG2b), CD11c-APC-Cy7 (N418, #117324, 1:40), CD8α-PerCP-Cy5.5(53-6.7, #100734, 1:50), CD40-Alexa Fluor® 647 (HM40-3, #102911, 1:40), CD80-PE (16-10A1, #104707, 1:20), CD86-PE-Cy7 (GL-1, #105013, 1:40), anti-MHC class I-Alexa Fluor® 647 (AF6-88.5.3, #116511, 1:40), anti-MHC class II-Pacific Blue (M5/114.15.2, #107620, 1:40), anti-IFN-γ-PE-Cy7 (XMG1.2, #505825, 1:40), and anti-TNF-α- PE-Cy7 (MP6-XT22, #506323, 1:40) were procured from BioLegend; anti-PD-L1 (B7-H1, #BE0101) were obtained from BioXcell (West Lebanon, NH, US). For the human Abs: isotype control Abs (IgG1, IgG2a or IgG2b), CD11c (3.9, #254813, 1:25), CD80 (2D10, #207831, 1:20), CD83 (HB15e, #147674, 1:40), CD86 (BU63, #202906, 1:40), anti-HLA-A, B, C (W6/32, #212641, 1:40), anti-HLA-DR, DP, DQ (Tü39, #211013, 1:40), anti-CD1c (L161, #331510, 1:40), anti-CD141 (M80, #344112, 1:40), and anti-IFN-γ (4S.B3, #193274, 1:40) were obtained from BioLegend (San Diego, CA, US).
Cd83 hb15e
Manufactured by BioLegend
CD83 (HB15e) is a cell surface protein that is expressed on mature dendritic cells. It plays a role in the activation and maturation of dendritic cells, which are important for initiating and regulating immune responses.
Automatically generated - may contain errors
Lab products found in correlation
Anti ifn γ pe cy7, by BioLegend (1 mentions)
W6 32, by BioLegend (1 mentions)
Cd80 2d10, by BioLegend (1 mentions)
Cd80 pe, by BioLegend (1 mentions)
Anti tnf α pe cy7 mp6 xt22, by BioLegend (1 mentions)
Cd11c apc cy7, by BioLegend (1 mentions)
Anti ifn γ 4s b3, by BioLegend (1 mentions)
Cd40 alexa fluor 647, by BioLegend (1 mentions)
Cd86 pe cy7, by BioLegend (1 mentions)
Cd11c, by BioLegend (1 mentions)
Cd45 hi30, by BD (1 mentions)
Cd3 sk7, by BD (1 mentions)
Cd66abce tet2, by Miltenyi Biotec (1 mentions)
Cd16 3ge, by BioLegend (1 mentions)
Cd56 hcd56, by BD (1 mentions)
Cd14 m5e2, by BD (1 mentions)
Cd1c ad5 8e7, by Miltenyi Biotec (1 mentions)
Cd11c b ly6, by BD (1 mentions)
Cd19 hib19, by BioLegend (1 mentions)
Cd20 l27, by BD (1 mentions)
2 protocols using cd83 hb15e
1
Comprehensive Immune Cell Profiling
2
Comprehensive Immune Phenotyping Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Phenotypic analysis was performed on NPA cells and PBMCs using Live/Dead Blue; lineage markers CD3 (SK7; BD), CD19 (HIB19; Biolegend), CD20 (L27, BD), CD45 (HI30; BD), CD56 (HCD56, BD) and CD66abce (TET2, Miltenyi Biotec); HLA-DR (TU36, Life Technologies), CD14 (M5E2, BD), CD16 (3GE, Biolegend), CD11c (B-Ly6, BD), CD1c (AD5-8E7; Miltenyi), CD141 (AD5-14H12; Miltenyi), CD123 (7G3; BD); maturation markers CD83 (HB15e, Biolegend) and CD86 (2331; BD); adhesion marker CD62L (SK11, BD); and migration markers CCR2 (K036C2, Biolegend) and CCR7 (150503: BD); and fixed with 1% paraformaldehyde for flow cytometry. Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences) and analysed using FlowJo software v10 (Tree Star).
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