Elisa kits for il 4 and tnf α

Ovalbumin (OVA), cholesterol and distearoyl phosphatidylcholine (DSPC) were acquired from Sigma-Aldrich, Inc. (St. Louis, MO). Lipidoid (C12-200) was purchased from Xinjiahecheng Medical Chemistry Corporation (Hubei, China). 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (mPEG-DMG) was purchased from NOF Corporation (Tokyo, Japan). The Giemsa staining kit for BALF was purchased from Baso Biotechnology Corporation (Guangdong, China).
Antibodies against F4/80 and CD206 were purchased from Santa Cruz Biotechnology (CA, USA). Antibodies against MBD2 and Arginase-1 were purchased from Abcam (MA, USA). The anti-GAPDH antibody was purchased from Proteintech (Hubei, China), while anti-Ym1 antibody was ordered from Thermo Fisher Scientific (PA, USA). PE-conjugated anti-human CD14, PE-conjugated anti-mouse F4/80, and APC-conjugated anti-mouse CD11c antibodies, ELISA kits for IL-4 and TNF-α were purchased from BioLegend (CA, USA).