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Em fc6

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The EM FC6 is a freezing unit designed for the preparation of cryo-ultrathin sections. It provides precise temperature control and efficient sample freezing for transmission electron microscopy (TEM) analysis.

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Lab products found in correlation

Jem 1010, by JEOL (2 mentions) Libra 120, by Zeiss (1 mentions) Lacey carbon coated copper grid, by Electron Microscopy Sciences (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Em afs2, by Leica (1 mentions) Tecnai f30 electron microscope, by Thermo Fisher Scientific (1 mentions) Tecnai g2 f20, by Thermo Fisher Scientific (1 mentions) Em 912 omega, by Zeiss (1 mentions) Osmium tetroxide, by Electron Microscopy Sciences (1 mentions) Xl30 microscope, by Philips (1 mentions)

Spelling variants of this product

EM UC6/FC6 ultramicrotome (6 citations) EM FC6 cryo-ultramicrotome (4 citations) Ultracut EM FC6 (3 citations) Leica EM UC6/FC6 cryo-microtome (2 citations) EM FC6 cryomicrotome (2 citations)

14 protocols using em fc6

1

Nanomaterials Characterization by AFM

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The materials used in this
study are IR rubber compounded with high-wear-resistance furnace-grade
CB (N330) and H-NBR rubber compounded with multiwall carbon nanotubes
(MWCNTs, C7000). Detailed formulations of these materials are listed
in Table S4. These materials are commercially
available.
To obtain a smooth surface suitable for AFM imaging,
we used a Leica EM FC6 (Leica Microsystems GmbH Wetzlar, Germany)
to perform ultrathin sectioning of the sample at −120 °C,
with the cutting direction perpendicular to the compression direction.
Afterward, we performed AFM measurements using a specially designed
sample holder for controlled compression.
Liang X., Liu H., Fujinami S., Ito M, & Nakajima K. (2024). Simultaneous Visualization of Microscopic Conductivity and Deformation in Conductive Elastomers. ACS Nano, 18(4), 3438-3446.
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2

Microstructural Analysis of Copolymer Synthesis

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Transmission electron microscopy (TEM) was used to analyse the microstructure of synthesized copolymers. Samples were trimmed at −60 °C using a cryo-ultramicrotome device, Leica EMFC6, Leica microsystems (Wetzlar, Germany), equipped with a diamond razor. The ultrathin sections (100 nm) were placed on 300 mesh copper grids. The surfaces were observed in a TECNAI G2 2o Twin (FEI, Hillsboro, OR, USA) operating at an accelerating voltage of 200 KeV in a bright-field image mode.
The welding zone of materials was analysed using scanning electron microscopy (SEM). TM3030Plus Tabletop Hitachi electron microscope (Hitachi High-Technologies Corporation, Fukuoka, Japan) was employed that operated at 15 kV in a standard (SD) observation mode. Before observation, the printed samples were cryo-fractured in liquid nitrogen and covered with a thin layer of gold using a Fine Coat Jeol Ion Sputter JFC-1100 (Peabody, MA, USA) to improve the electron conductivity during the SEM measurements.
Peñas M.I., Calafel M.I., Aguirresarobe R.H., Tierno M., Conde J.I., Pascual B, & Santamaría A. (2020). How Is Rheology Involved in 3D Printing of Phase-Separated PVC-Acrylate Copolymers Obtained by Free Radical Polymerization. Polymers, 12(9), 2070.
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3

Rubber Composite Surface Characterization

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The material used
in this study was a rubber composite with 100 phr of IR and 40 phr
of high-abrasion furnace grade CB (N330, mean particle size of 84
nm); the detailed composition of this material is shown in Table 1. All raw materials
were commercially available. To obtain a smooth material surface for
AFM imaging, the samples were ultramicrotomed at −120 °C
using a Leica EM FC6 (Leica Microsystems GmbH Wetzlar, Germany), with
a cutting direction perpendicular to the compression direction. AFM
measurements under controlled compression were then performed by using
a specially designed sample holder.
Liang X., Kojima T., Ito M., Amino N., Liu H., Koishi M, & Nakajima K. (2023). In Situ Nanostress Visualization Method to Reveal the Micromechanical Mechanism of Nanocomposites by Atomic Force Microscopy. ACS Applied Materials & Interfaces, 15(9), 12414-12422.
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4

TEM Analysis of Filler Dispersion

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The digital image analysis of filler dispersion through TEM was carried out following three steps: (1) the vulcanized compound was sliced with a diamond cryo-cutter under liquid nitrogen to obtain sections with thicknesses around 100 nm, (2) sections were observed in the transmission electron microscope and 10 images were acquired with a digital camera for each sample, and (3) the acquired images were visually analyzed to determine the amount of undispersed filler particles.
TEM characterizations were performed with a TEM, LIBRA® 120, Zeiss, Oberkochen (Baden-Württemberg, Germany), with an acceleration voltage of 120 kV.
Ultra-thin sections must be prepared from the sample material in order to analyze it using the TEM. For this purpose, a cryo-ultramicrotome (type: Leica EM FC 6, Leica Microsystem, Wetzlar, Germany) using a diamond knife (type: Diatome 35°) at a temperature of −80 °C was used to produce sections with a width approx. 100 µm and a thickness approx. 100 nm. The preparation was carried out using the wet-cutting method, in which the sections are floated on a mixture of dimethyl sulphoxide (DMSO) and water (50/50) and transferred to a copper mesh using a loop. To stabilise the sections in the transmission electron microscope, they were placed on a 400 mesh copper carrier coated with poly(vinyl formal) (Plano).
Magaletti F., Galbusera M., Gentile D., Giese U., Barbera V, & Galimberti M. (2024). Carbon Black Functionalized with Serinol Pyrrole to Replace Silica in Elastomeric Composites. Polymers, 16(9), 1214.
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5

Characterizing Rubber-Carbon Black Nanocomposites

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The PNCs in this study were a mixture of isoprene rubber and high-abrasion furnace-grade CB (N330, with a mean particle size of 28–36 nm). Its formulations are presented in Table 1. All ingredients were commercially available. The loading contents of CB were 10, 20, 30, 40, and 50 phr, which corresponded to 4.9%, 9.1%, 13.2%, 16.7%, and 20.2% in volume fraction, respectively. A specially designed sample holder was used to maintain the CB/IR sheets (20 mm × 2 mm × 0.2 mm) in a uniaxially stretched condition under the AFM probe. Prior to stretching, two lines normal to the stretching direction were marked on the surface of the samples. The macroscopic strain can be precisely calculated from the measurement of the distance between two lines. Then, the deformed samples were ultramicrotomed using a Leica EM FC6 (Leica Microsystems GmbH Wetzlar, Germany) at −120 °C to obtain a smooth surface for AFM imaging. Cutting was performed parallel to the tensile direction.
Liang X., Ito M, & Nakajima K. (2021). Reinforcement Mechanism of Carbon Black-Filled Rubber Nanocomposite as Revealed by Atomic Force Microscopy Nanomechanics. Polymers, 13(22), 3922.
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6

Cryo-Microtoming for High-Resolution STEM

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Thin sections with thickness of ∼120 nm were obtained by cryo-microtoming at −120 °C using a Leica EM FC6 and picked up on a lacey carbon coated copper grid (Electron Microscopy Sciences). Scanning transmission electron microscopy experiments were performed on a Tecnai F20 UT FEG, equipped with a high-angle annular dark-field detector, using 200 keV acceleration voltage.
Petzetakis N., Doherty C.M., Thornton A.W., Chen X.C., Cotanda P., Hill A.J, & Balsara N.P. (2015). Membranes with artificial free-volume for biofuel production. Nature Communications, 6, 7529.
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7

Nanocomposite Morphology Imaging by TEM

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The structural morphologies of the nanocomposites were imaged with
TEM using the JEM-1010, JEOL instrument at an acceleration voltage
of 80 kV. For imaging, thin slices of the sample of thickness ∼120
nm were obtained by cryomicrotoming at −120 °C using a
Leica EM FC6 instrument and were anchored on carbon-coated copper
grids.
Sattar M.A, & Patnaik A. (2020). Role of Interface Structure and Chain Dynamics on the Diverging Glass Transition Behavior of SSBR-SiO2-PIL Elastomers. ACS Omega, 5(33), 21191-21202.
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8

Cryosectioning and Immunogold Labeling of Parasites

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For HA-tagged GyrA, SSB or Prex, free tachyzoites were recovered following egress from HFF cultures and fixed for 2 h with 4% freshly prepared formaldehyde and 0.05% glutaraldehyde in PBS. Parasites were washed with PBS and the parasite pellets were embedded in a 10% gelatin solution and infiltrated by immersing in 2.3 M sucrose at 4 °C overnight. The infiltrated parasite pellets were trimmed, mounted and flash frozen by immersion in liquid nitrogen. Ultrathin sections (70–80 nm) were obtained using a cryo-ultramicrotome (Leica EM FC6), collected with 2.3 M sucrose and mounted on copper grids covered with a formvar film. The ultrathin sections were rehydrated in 2% gelatin, blocked with 3% BSA (Sigma-Aldrich) in PBS and labelled with rat anti-HA antibody for 1 h followed by 10 nm gold conjugated goat anti-rat IgG (Sigma-Aldrich). Sections were stained with 0.5% uranyl acetate in 2% methylcellulose and observed on a Zeiss 900 transmission electron microscope.
Martins-Duarte É.S., Sheiner L., Reiff S.B., de Souza W, & Striepen B. (2021). Replication and partitioning of the apicoplast genome of Toxoplasma gondii is linked to the cell cycle and requires DNA polymerase and gyrase. International Journal for Parasitology, 51(6), 493-504.
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9

Ultrastructural Imaging of Frozen Embryos

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For EM analysis, the embryos were high-pressure frozen (HPM010 AbraFluid), using 20% dextran as cryoprotectant. The embryos were pierced with a needle in a cryo-microtome chamber (Leica EM FC6) at −160 °C to facilitate freeze substitution53 (link). Embryos were then freeze-substituted (EM-AFS2 - Leica Microsystems) with 0.3% Uranyl Acetate (UA), 0.3% Glutaraldehyde and 3% water in acetone at −90 °C for 48 h. The temperature was then raised to −45 °C at 2 °C/h and samples were further incubated for 16 h. After rinsing in acetone, the samples were infiltrated in Lowicryl HM20 resin, while raising the temperature to −25 °C and left to polymerize under UV light for 48 hours at −25 °C and for further 9 hours while the temperature was gradually raised to 20 °C(5 °C/h). 70 nm cross sections were cut from the polymerized resin block in the region of the embryo cauterization and picked up on formvar coated slot grids. Tiled 2D images were acquired with a FEI Tecnai F30 electron microscope. Images were then stitched using Etomo (Imod software package54 (link)).
de Medeiros G., Kromm D., Balazs B., Norlin N., Günther S., Izquierdo E., Ronchi P., Komoto S., Krzic U., Schwab Y., Peri F., de Renzis S., Leptin M., Rauzi M, & Hufnagel L. (2020). Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Scientific Reports, 10, 1942.
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10

Characterization of SiO2-Filled Natural Rubber Nanocomposites

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The surface area and porosity of the SiO2 filler were
measured by the Brunauer–Emmett–Teller (BET) technique
using a Quantachrome Nova2000e series surface area analyzer. The structural
morphology of the composites was unraveled with transmission electron
microscopy (TEM) with a JEM-1010, JEOL instrument at an acceleration
voltage of 80 kV. Thin slices of the samples with a thickness of ∼120
nm were obtained by cryo-microtoming at −120 °C using
a Leica EM FC6 instrument and were transferred onto carbon-coated
copper grids. Following this, the interaction between SiO2 and NR in the nanocomposites was studied with a Spectrum Two PerkinElmer
FTIR spectrophotometer by acquiring spectra at 4 cm–1 resolutions, averaged over 32 scans. An FT-Raman spectrometer (Bruker
RAMII) working in a confocal mode, connected to a Leica microscope,
was used for the measurement of the Raman spectra. The laser beam
was focused by a 100× magnification objective of a confocal microscope.
Each spectrum was collected in the frequency range 100–3500
cm–1 over 60 s and with 10 accumulations to avoid
electronic peaks and average background. Thermogravimetric analyses
(TGA) were performed with a TGA Discovery series, TA Instruments,
New Castle, DE machine. Samples were heated over a temperature range
of 22 °C (room temperature) to 900 °C at a heating rate
of 20 °C/min under a N2 atmosphere.
Sattar M.A., Gangadharan S, & Patnaik A. (2019). Design of Dual Hybrid Network Natural Rubber–SiO2 Elastomers with Tailored Mechanical and Self-Healing Properties. ACS Omega, 4(6), 10939-10949.
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