Histidine

Manufactured by HiMedia

Sourced in India

Histidine is an essential amino acid that is commonly used in various laboratory applications. It is a neutral, polar amino acid that plays a crucial role in the structure and function of proteins. Histidine is a key component in the active sites of many enzymes and is involved in the regulation of pH within biological systems.

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3 protocols using histidine

Synthesis and Characterization of Biomolecule-Functionalized Nanoparticles

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All of the chemicals used in this work were analytical grade. Chloroauric acid (HAuCl₄), silver nitrate (AgNO₃) and spectroscopic grade potassium bromide (KBr) were purchased from Sigma-Aldrich (ACS reagent, ≥99.99%, St. Louis, MA, USA). Sodium citrate (Na₃C₆H₅O₇) was used as received from Merck (KGaA Darmstadt, Germany). Urea, histidine, tryptophan, glutamic acid, creatinine, l-cysteine, methionine, glutathione, threonine, phenylalanine, arginine and proline were obtained from Hi-Media (AR reagent, 99.0% Mumbai, India). Ultrapure water (18.2 MΩ cm) was used for preparing all of the solutions. The stock solutions (1000 μg mL⁻¹) of all the biomolecules (i.e., amino acids) were prepared by dissolving a proper quantity of substance in ultrapure water. The working solutions were prepared by the appropriate dilution of the standard stock solution.

Saha A., Khalkho B.R, & Deb M.K. (2021). Au–Ag core–shell composite nanoparticles as a selective and sensitive plasmonic chemical probe for l-cysteine detection in Lens culinaris (lentils). RSC Advances, 11(33), 20380-20390.

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Comprehensive Reagents and Cell Lines

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Unless otherwise mentioned all chemicals used are of the highest quality available and purchased from Sigma Aldrich (St. Louis, MO, USA). agarose, agar, ethidium bromide, catalase, m ethanol, ethanol, isopropanol, DMSO, NaCl, EDTA, Tris-HCl, Triton-X 100, NaOH, paraformaldehyde, bovien serum albumin (BSA), biotin, histidine, ampicillin, oxoid nutrient broth No. 2, and protease inhibitor cocktail were obtained from HiMedia (Mumbai, India), sodium fluoride and orthovandate from MP Biomedicals, LLC (Solon Ohio, USA), CellROX Deep Red and Nuc Blue (Hoechst 33342) from ThermoFisher Scientific (Santa Clara, CA), Salmonella typhimurium TA100 from Microbial Type Culture Collection and Gene Bank (MTCC, Chandigarh, India), thalidomide and DMEDA from Tokyo Chemical Industry (Tokyo, Japan), nitrocellulose membranes from MDI Membrane Technologies (Ambala. India), LMPA and LE agarose from Lonza (Rockland, ME, USA), γ-H2AX Alexa Fluor 488 from Biolegend (San Diego, CA, USA), and PAD-PARP antibody from Abcam (MA, USA). The 293T cell line was purchased from National Centre for Cell Culture (Pune, India) and authenticated by Short Tandem Repeat DNA profiling from Life Code Technology, Delhi, India. HepG2 cells were a generous gift from Dr. Soumya Sinha Roy at the CSIR Institute of Genomics and Integrative Biology, New Delhi. HUVEC was procured from HiMedia (Mumbai, India).

Wani T.H., Chakrabarty A., Shibata N., Yamazaki H., Guengerich F.P, & Chowdhury G. (2017). The Dihydroxy Metabolite of the Teratogen Thalidomide Causes Oxidative DNA Damage. Chemical research in toxicology, 30(8), 1622-1628.

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Bacterial Strain Cultivation and Amino Acid Preparation

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The bacterial strain Xanthomonas citri pv. glycines 12-2, a causal agent of bacterial pustule of soybean was used in the study. Bacterial cells were stored in nutrient broth (NB) medium supplemented with glycerol (25%) and maintained at -80 °C. X. citri pv. glycines 12-2 was cultured at 28 °C in nutrient broth (NB) or nutrient agar (NA) for routine work or in the minimal medium M9 (Sambrook et al., 1989) . Escherichia coli DH5 strains were routinely grown in LB medium at 37 °C.
Eleven amino acids were tested in the study: aspartic acid, glutamic acid, asparagine, glutamine, lysine, threonine, arginine, serine, proline, leucine, histidine, alanine, methionine, isoleucine, cysteine, and valine, were purchased from HiMedia Laboratories LLC. Stock solutions of all amino acids were prepared at a concentration of 200 mM in sterilized de-ionized water and filter sterilized through a 0.22-μm-pore-size filter.

Pluemjit N., Sripo‐ngam S., Khwanbua E., & Chatnaparat T. (2020). Role of Amino Acid Cysteine in the Suppression of Xanthomonas citri pv. glycines Bacterial Pustule Disease of Soybean. Chiang Mai University Journal of Natural Sciences, 19(4).

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