Pm100a s120vc

Analog signals were sampled at 10 kHz, amplified 100 times, high‐pass filtered at 0.1 Hz, (±0.1 V input range and 1.526 μV/bit input‐referred resolution) using a USB‐6259 NI‐DAQ card (National Instruments). The NI‐DAQ card was connected to a computer that was used to control stimulation and data acquisition using custom‐made software. A TTL‐controlled laser (SLOC Lasers) was used to deliver blue light (473 nm) through the optrode. Prior to implantation, the laser output settings were defined to achieve four increasing light power densities (LPDs) at the optical fiber tip: 64, 127, 191, and 255 mW/mm². This was checked using a handheld light power meter (PM100A + S120VC; ThorLabs). A constant‐current linear stimulus isolator (Digitimer) generated the charge‐balanced square‐wave pulses (200 μs per phase) needed to obtain the EPs and afterdischarges.

Analog signals were high-pass filtered at 0.1 Hz, amplified 248 times, and digitized at a 16-bit resolution (±10 V input range) using a USB-6259 NI-DAQ card (National Instruments, Austin, TX, United States). The NI-DAQ card was connected to a computer which was used to control the stimulation and data acquisition using custom-made software. CA1 EPs were sampled at 10 kHz. A TTL-controlled laser (SLOC Lasers, China) was used to deliver blue light (473 nm) through the optic fiber of the optrode. Prior to implantation, laser output was adjusted to achieve the light-power density (LPD) levels 32, 160, and 320 mW/mm² at the optic fiber tip. This was achieved using a handheld light power-meter (PM100A + S120VC, ThorLabs, Germany). A constant-current linear stimulus isolator (Digitimer, United Kingdom) generated charge-balanced square-wave pulses (200 μs per phase) needed to obtain the EPs.