Model leo 912 omega

Manufactured by Zeiss

Sourced in Germany

The Zeiss LEO 912 Omega is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials at the nanoscale. It features an electron beam that is accelerated to high energies, allowing for the examination of thin samples with a high degree of detail and resolution.

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LEO 912 Omega LEO 912 912 Omega

2 protocols using model leo 912 omega

TEM Analysis of Cellulose Nanofibers

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Morphology and dimensions of the cellulose nanofibers were investigated by transmission electron microscopy (TEM). Drops of a 0.001% (w/v) CNF suspension were deposited on carbon coated copper grids (CF400-CU Carbon Film, 400 Mesh Copper grids), which were previously cleaned with plasma to get rid of surface contamination and make the grid more adhesive towards the sample. The samples were negatively stained with 2% (w/v) uranyl acetate in suspension. Then, samples were allowed to dry and were observed in the TEM microscope, model Zeiss LEO 912 Omega (Carl Zeiss Microscopy GmbH, Jena, Germany), operating at 80 kV.

Kamdem Tamo A., Doench I., Walter L., Montembault A., Sudre G., David L., Morales-Helguera A., Selig M., Rolauffs B., Bernstein A., Hoenders D., Walther A, & Osorio-Madrazo A. (2021). Development of Bioinspired Functional Chitosan/Cellulose Nanofiber 3D Hydrogel Constructs by 3D Printing for Application in the Engineering of Mechanically Demanding Tissues. Polymers, 13(10), 1663.

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Characterizing Nanoparticle Morphology by TEM

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The size, shape, and surface structure of nanoparticles were characterized using transmission electron microscopy (TEM). The centrifugate of the chitosan–caseinate PEC system was washed several times with distilled water. Thereafter a droplet containing the washed chitosan–caseinate centrifugate (5 µL) was deposited on carbon coated copper grids (CF400-CU Carbon Film, 400 Mesh Copper grids) which were previously cleaned with plasma to get rid of surface contamination and make the grid more adhesive towards the sample, and allowed to dry for 1 min. Excess solution was wiped off and the sample was negatively stained with 3 µL of uranyl acetate for 2 min. The samples were observed with TEM microscope, model Zeiss LEO 912 Omega (Carl Zeiss Microscopy GmbH, Jena, Germany).

Lall A., Kamdem Tamo A., Doench I., David L., Nunes de Oliveira P., Gorzelanny C, & Osorio-Madrazo A. (2020). Nanoparticles and Colloidal Hydrogels of Chitosan–Caseinate Polyelectrolyte Complexes for Drug-Controlled Release Applications. International Journal of Molecular Sciences, 21(16), 5602.

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