Propionic acid

Manufactured by Genesee Scientific

Propionic acid is a colorless liquid organic compound with a pungent odor. It is a naturally occurring short-chain fatty acid that can be used in various industrial and scientific applications.

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Lab products found in correlation

Tegosept, by Genesee Scientific (2 mentions) Nutri fly bloomington formulation fly food, by Genesee Scientific (1 mentions) D glucose, by Merck Group (1 mentions) Np6293 gal4, by Kyoto Stock Center (1 mentions) W1118, by Bloomington Drosophila Stock Center (1 mentions) Uas dicer2, by Vienna Drosophila Resource Center (1 mentions) Inactive dry yeast, by Genesee Scientific (1 mentions) Uas ecr rnai, by Bloomington Drosophila Stock Center (1 mentions) Uas mcd8 gfp, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using propionic acid

Rearing and Infecting Drosophila melanogaster

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For all experiments, wild-type Canton S Drosophila melanogaster (BDSC stock no. 1, obtained from the Lazzaro lab at Cornell University) were used. To rear age-matched adults, 10 males and 10 females were placed into plastic vials with approximately 5 mL of Nutri-fly Bloomington formulation fly food (Genesee Scientific, San Diego, CA). To prevent fungal and microbial growth, 4.8 mL of 1 M propionic acid and 1 g Tegosept (Genesee Scientific, San Diego CA) suspended in 10 mL of ethanol were added to 1 L of prepared food after the food cooled to approximately 40°C. Flies laid eggs for two days before adult flies were emptied from the vials. Ten days after adult flies were removed, newly eclosed adult flies (0 to 2 days old) were sorted in vials of 10 males. These flies were housed for an additional 5 days before infection procedures to allow their immune systems to fully develop so that experiments were conducted with 5 to 7-day-old adult flies. Throughout rearing and experiments, all flies were kept in a 25°C incubator with a 12 h light-dark cycle.

Wukitch A.M., Lawrence M.M., Satriale F.P., Patel A., Ginder G.M., Van Beek E.J., Gilani O, & Chambers M.C. (2023). Impact of Chronic Infection on Resistance and Tolerance to Secondary Infection in Drosophila melanogaster. Infection and Immunity, 91(3), e00360-22.

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Drosophila Rearing and Strain Collection

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All flies were raised at 25°C under 12 hours/12 hours light/dark cycle. The animals were reared on standard fly food containing 6 g Drosophila agar type II (Genesee Scientific, #66–103), 100 g D-(+)-glucose (SIGMA, #G8270–25KG), 50 g inactive dry yeast (Genesee Scientific, #62–106), 70 g yellow corn meal (Genesee Scientific, #62–101), 6 ml propionic acid (SIGMA, #402907–500ML), and 10 ml Tegosept (Genesee Scientific, #20–258) in 1,025 ml of water. Flies used are as follows: w¹¹¹⁸ (#5905; the control strain), Mi{MIC}Oatp74D^MI05878 (#42353), EcRE-LacZ (#4516 and #4517), UAS-EcR RNAi (#9327), and UAS-mCD8-GFP (#5130) were obtained from the Bloomington Drosophila Stock Center (BDSC); UAS-dicer2 (#60008 and #60009) was obtained from the Vienna Drosophila Resource Center (VDRC); UAS-EcI (Oatp74D) RNAi (#7571R-1) was obtained from the National Institute of Genetics Fly Stock Center; NP2276-Gal4 (#112853) and NP6293-Gal4 (#105188) were obtained from the Kyoto Stock Center (Department of Drosophila Genomics and Genetic Resources, Kyoto Institute of Technology). 9–137-Gal4 and Moody-Gal4 were obtained from Ulrike Heberlein (Janelia Research Campus) and Ulrike Gaul (Ludwig-Maximilians-Universität München), respectively.

Okamoto N, & Yamanaka N. (2020). Steroid Hormone Entry into the Brain Requires a Membrane Transporter in Drosophila. Current biology : CB, 30(2), 359-366.e3.

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