Swabs and internal organs were streaked onto MacConkey agar, sorbitol MacConkey agar (SMA), and incubated aerobically at 37°C for 24 h. Lactose-fermenting colonies (pink colonies) on MacConkey agar and white colonies on SMA were selected and subcultured on brain heart infusion agar for purification. The purified colonies were subjected to Gram staining and oxidase test. Colonies were oxidase-negative, and Gram-negative bacilli were identified biochemically to the species level by the VITEK® 2 COMPACT system using GN identification cards (BioMérieux, France).
Gn identification cards
Manufactured by bioMérieux
Sourced in France
The GN identification cards are a diagnostic tool used in microbiology laboratories for the identification of Gram-negative bacteria. The cards provide a standardized method for bacterial identification, allowing for reliable and consistent results.
Automatically generated - may contain errors
2 protocols using gn identification cards
1
Bacterial Identification from Clinical Samples
2
Isolation and Identification of Pseudomonas from Water Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The microfuge tubes and water samples were gently vortexed and an aliquot of 10 μL was streaked onto pseudomonas cetrimide agar (PCA) and brain heart infusion (BHI) agar (Oxoid, Basingstoke, UK) and incubated aerobically at 37 °C for 24 h. Growths were examined for colony morphology, Gram staining, and the oxidase test. Gram-negative isolates that were oxidase-positive were subjected to further biochemical identification by the Vitek 2 compact system using GN identification cards (BioMérieux, Marcy L’Etoile, France).
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