Male Sprague–Dawley rats (Crl: SD; Charles River, Wilmington, MA) aged 20 weeks, male and female C57BL/6J mice aged 8 to 20 weeks (Jackson), male BKS.Cg-Dock7m+/+Leprdb/J aged 16 weeks (BKS.Leprdb/+, Jackson),17 (link) male athymic nude mice aged 18 weeks (Fox1nu; Envigo, Indianapolis, IN), and male BALB/cJ mice aged 12 weeks (Jackson) were used in this study. Rats were housed in groups of 3 and mice in groups of 4 to 5. Animals were maintained on a 12-hour light–dark cycle with ambient temperatures between 20 and 22°C, with food and water freely available. Animal protocols were approved by the Institutional Animal Care and Use Committee of the University of Texas Health Science Center at San Antonio and conformed to International Association for the Study of Pain and federal guidelines.
Fox1nu
Manufactured by Inotiv
Sourced in India, United States
The Fox1nu is a laboratory instrument used for the analysis and detection of specific gene targets. It is designed to perform sensitive and accurate nucleic acid amplification reactions, such as real-time PCR (Polymerase Chain Reaction). The core function of the Fox1nu is to facilitate the identification and quantification of target genetic sequences in a sample.
Automatically generated - may contain errors
3 protocols using fox1nu
1
Animal Models for Biomedical Research
2
Antitumor Efficacy of 3-Ga Nanoparticles
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Nude mice (FOX1nu, Envigo, Indianapolis, IN, USA) handling
was performed according to the guidelines of the National Institutes
of Health (NIH) and the Association for Assessment and Accreditation
of Laboratory Animal Care (AAALAC). The study was conducted in one
cycle. On the first day of the study, mice were injected into their
right flank with 106 DU-145 cells in 100 μL of PBS:Matrigel
(1:1, Life Sciences, Chicago. IL, USA). Tumor volume was first determined
upon tumor appearance and twice a week thereafter until study termination.
Once tumors reached a volume of 85–115 mm3, mice
were distributed into six groups with similar mean tumor volume, consisting
of seven or nine mice per group (in accordance with the displayed
experiment: n = 7 or n = 9). IV
treatments were initiated with3-Ga NPs for 2 weeks in
accordance with the timeline depicted inScheme 1 . Following treatment, mice were monitored
for body weight and tumor size for two additional weeks. Before termination,
animals were weighed, tumor volumes were determined, and mice were
sacrificed by CO2 asphyxiation followed by tumor excision,
weighing, and fixation in formalin.
was performed according to the guidelines of the National Institutes
of Health (NIH) and the Association for Assessment and Accreditation
of Laboratory Animal Care (AAALAC). The study was conducted in one
cycle. On the first day of the study, mice were injected into their
right flank with 106 DU-145 cells in 100 μL of PBS:Matrigel
(1:1, Life Sciences, Chicago. IL, USA). Tumor volume was first determined
upon tumor appearance and twice a week thereafter until study termination.
Once tumors reached a volume of 85–115 mm3, mice
were distributed into six groups with similar mean tumor volume, consisting
of seven or nine mice per group (in accordance with the displayed
experiment: n = 7 or n = 9). IV
treatments were initiated with
accordance with the timeline depicted in
for body weight and tumor size for two additional weeks. Before termination,
animals were weighed, tumor volumes were determined, and mice were
sacrificed by CO2 asphyxiation followed by tumor excision,
weighing, and fixation in formalin.
3
Profiling Mutational Landscape of HCC Cell Lines
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The animal study protocol was approved by the institutional animal care and use committee (IACUC) of the Icahn School of Medicine at Mount Sinai (IACUC‐2016‐0039). Female athymic nude mice (Fox1nu) of 6‐10 weeks were purchased from Envigo. Three different human HCC cell lines were used that were derived from previous reports: Huh7, HepG2, and green fluorescent protein (GFP)‐micro‐RNA (miR)‐517a‐Huh7.(17 , 18 ) Cell lines were cultured as monolayers in Dulbecco's Modified Eagle’s Medium supplemented with 10% fetal bovine serum, 1% penicillin‐streptomycin, and 1% L‐glutamine, at 37°C. IMPACT I (It is a test that includes PCR evaluation for: Ectromelia, EDIM, Hantaan, K virus, LCMV, LDEV, MAV1, MAV2, mCMV, MHV, MNV, MPV, MTV, MVM, Mycoplasma pulmonis, Mycoplasma sp., Polyoma, PVM, REO3, Sendai, TMEV) was tested by IDEXX BioResearch in each cell line to exclude contamination. DNA was extracted from the cell lines with the DNeasy Blood and Tissue Kit (Qiagen) and submitted to ultra‐deep DNA sequencing of a target panel of 58 frequently mutated genes in HCC, as described.(6 )
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