Anti hif 1α 610958

IDF-11774 were synthesized as described previously.^{18 (link)} Sunitinib and 5-FU were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sorafenib and lapatinib were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Stock solutions of compounds were prepared in DMSO at 10 mM and stored at −20 °C. The following antibodies were used: anti-β-actin (sc-47778), anti-ACCα (sc-30212), and anti-AMPKα (sc-25792) from Santa Cruz Biotechnology; anti-HER2 (2242), anti-mTOR (2983), anti-p-mTOR (2971), anti-pACC (3661), anti-4EBP1 (9644), anti-p-4EBP1 (9459), and anti-p-AMPK (2531) from Cell Signaling Technology (Beverly, MA, USA); anti-HIF-1α (610958) and anti-cyclin D1 (554180) from BD Biosciences (San Diego, CA, USA); anti-β-tubulin (ab15568) from Abcam (Cambridge, MA, USA).

Cycloheximide, 5-aza-2′-deoxycytidine (5-Aza), trichostatin A (TSA), and rapamycin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The indicated primary antibodies against the following proteins were used in this study: STAT1/2/3/4/5/6 (#9172/4594/12640/5097/9363/9362), DNMT1/3a/3b (#5119/3598/67259), cleaved-caspase 3/7 (#9664/9491), cleaved PARP (#5625), S6K (#2708), phospho-S6K (T389) (#9205), S6 (#2217), phospho-S6 (Ser235/236) (#2211), mTOR (#2972), 4E-BP1 (#9644), phospho-4E-BP1 (T70) (#9455), eIF4E (#2067), phospho-eIF4E (S209) (#9741), eIF4G (#2469), Rheb (#13879), TSC2 (#4308), p-TSC2 (T1462) (#3617), p-TSC2 (S1387) (#5584), ICAM1 (#4915), JAK2 (#3230), NFATC2 (#4389) and Myc taq (#2278) (Cell Signaling Technology, Danvers, MA, USA); anti-HIF-1α (610958) (BD Biosciences, San Jose, CA, USA); and anti-actin (sc-1616) and GAPDH (sc-48,167) (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Secondary antibodies used were anti-goat IgG HRP (81–1620; Invitrogen, Carlsbad, CA, USA), anti-mouse IgG HRP (G-21040; Invitrogen), and anti-rabbit IgG HRP (111–035-003; Jackson Laboratories, Bar Harbor, ME, USA).