All experimental procedures were approved by the Mayo Clinic Institutional Animal Care and Use Committee and performed in accordance with NIH guidelines. Adenosine A2A receptor (A2AR: Adora2A, MMRRC:031168-UCD)-Cre mice were provided by Dr. Jiang-Fan Chen at Wenzhou Medical University. Aldehyde dehydrogenase 1 family, member L1 (ALDH1L1)-Cre (Stock #023748), Dopamine D2 receptor (D2R: DRD2)-eGFP (stock #030255), Dopamine D1 receptor (D1R: DRD1)-tdTomato (stock #016204) and C57BL/6J mice were purchased from Jackson Laboratory (Bar Harbor, ME). The ENT1 KO mice were generated as described (27 (link)). Mice were housed in standard Plexiglas cages and the colony room was maintained at a constant temperature (24 ± 1°C) and humidity (60 ± 2%) with a 12 h light/dark cycle (lights on at 07:00 A.M.). We used 8- to 10-wk-old male mice for all experiments.
Drd1 tdtomato
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
DRD1)-tdTomato is a fluorescent fusion protein that consists of the dopamine receptor D1 (DRD1) fused to the red fluorescent protein tdTomato. It is designed for visualizing the localization and expression of the DRD1 receptor in live cells.
Automatically generated - may contain errors
2 protocols using drd1 tdtomato
1
Transgenic Mouse Models for Neurological Research
2
Transgenic Mouse Models of Receptor Signaling
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Animal procedures were conducted in accordance with the NIH Guidelines for the Care and Use of Experimental Animals and were approved by the Institutional Animal Care Committee. The Nr4a1-eGFP hemizygous, Nr4a1-null (JAX Nr4a1tm1Jm #006187), Drd1-eGFP [Tg(Drd1a-eGFP)X60Gsat], and Drd1 tdTomato (catalog #016204, The Jackson Laboratory) mice used for this study were obtained from GENSAT and The Jackson Laboratory, respectively. Controls always consisted of wild-type littermates. Mice were given ad libitum access to food and water and housed under a 12 h light/dark cycle. Only male mice were used in these studies.
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