Rna from torula yeast

Commercially available solid (powder) reference samples of: cholesterol (Sigma), melanine from Sepia officinalis (Sigma), mannan from Saccharomycs cerevisiae (Sigma), DNA from salmon testes (Sigma), RNA from torula yeast (Sigma), galactoseamine (Merck), N-acetyl galactoseamine (Merck), soy phospholipids (Merck), as well as building blocks of DNA and RNA, i.e., uracil (Merck), thymine (Koch), adenine (CHR), guanine (Sigma), and cytosine (Sigma) were all analyzed at room temperature, without further purification, and after gentle homogenization in a mortar.
Lysogeny Broth (LB) was prepared by adding 10 g/L of Tryptone (Fluka Analytics), 1 g/L of select yeast extract (Sigma life science), 8 g/L of NaCl (≥99.5% purity, Sigma-Aldrich Chemie GmbH), 0.3 g/L of CaCl₂ (97% purity, Fluka Analytics), 1 g/L Dextrose (Biotechnology grade, Amresco) and 2 mg/L Streptomycin (Fluka Chemie GmbH) in ultra-pure water.
The virus dilution buffer (i.e phosphate buffered saline, PBS) was prepared by adding 0.78 g/L NaH₂PO₄ 2H₂O (≥98.0% purity, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) and 0.58 g NaCl (≥99.5% purity, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) in ultra-pure water. The pH was equilibrated to seven using NaOH (≥99% purity, Carl Roth GmbH, Karlsruhe, Germany) and HCl (ACS reagent grade, Sigma-Aldrich, Buchs, Switzerland).

Ext.DrewAT, DrewAT, Ds26, misTA, allAT, and allCG30 31 (link) were obtained from Integrated DNA Technologies (Coralville, IA, USA) (Table S2). The autocomplementary oligonucleotides (DrewAT, Ext.DrewAT, Ds26, allAT, and allCG) were heated at 95 °C for 5 min in milliQ water and then slowly cooled to room temperature to generate double-stranded DNA structures. Concentrations were determined by measuring UV-Vis absorption using the supplier’s values for the molar extinction coefficients at 260 nm. DNA sodium salt from calf thymus (CT-DNA) and RNA from torula yeast were purchased from Sigma-Aldrich (St. Louis, MO, USA). CT-DNA and RNA were dissolved in 10 mM sodium phosphate buffer (pH 7.4, 100 mM NaCl) and filtered. Concentrations were evaluated by UV considering that an OD 260 nm of 1 corresponds to approximately 50 μg/mL double-stranded DNA and 40 μg/mL RNA, respectively.