Bioplex bead array technique

Manufactured by Bio-Rad

The Bio-Rad Bioplex Bead Array technique is a multiplex assay system that enables the simultaneous detection and quantification of multiple analytes in a single sample. The core function of this technique is to provide a flexible and high-throughput platform for the analysis of proteins, cytokines, and other biomolecules.

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Lab products found in correlation

Multiplex cytokine panel assay, by Bio-Rad (3 mentions)

3 protocols using bioplex bead array technique

Multiplex cytokine quantification in BAL

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Cytokine concentrations in BAL fluid were quantified using the Bioplex Bead Array technique and a multiplex cytokine panel assay (Bio-Rad Laboratories, Hercules, CA) as previously described [3 (link)].

Stone M.L., Zhao Y., Robert Smith J., Weiss M.L., Kron I.L., Laubach V.E, & Sharma A.K. (2017). Mesenchymal stromal cell-derived extracellular vesicles attenuate lung ischemia-reperfusion injury and enhance reconditioning of donor lungs after circulatory death. Respiratory Research, 18, 212.

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Multiplexed Cytokine Profiling in Rat Aortic Tissue

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Cytokine content in rat aortic tissue homogenates was quantified using the Bio-Plex bead array technique using a multiplex cytokine panel assay (Bio-Rad Laboratories, Hercules, CA). Tissue inhibitor of metalloproteinase 1 (TIMP-1) concentrations were determined in terms of mean pixel intensity using a Proteome Profiler Array Rat Cytokine Array Panel A (R&D Systems, Inc, Minneapolis, MN), and array data were developed on x-ray film and quantified using Image Lab software (Version 3.0; Bio-Rad Laboratories, Hercules, CA). Western blot analyses for uPA, tPA, and PAI-1 protein concentrations were performed in a standard fashion.

English S.J., Piert M.R., Diaz J.A., Gordon D., Ghosh A., D'Alecy L.G., Whitesall S.E., Sharma A.K., DeRoo E.P., Watt T., Su G., Henke P.K., Eliason J.L., Ailawadi G, & Upchurch GR J.r. (2015). Increased 18F-FDG Uptake Is Predictive of Rupture in a Novel Rat Abdominal Aortic Aneurysm Rupture Model. Annals of surgery, 261(2), 395-404.

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Quantification of Cytokines and HMGB1

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Cytokine content in murine aortic tissue homogenates and cell culture supernatants was quantified using the Bioplex Bead Array technique using a multiplex cytokine panel assay (Bio-Rad Laboratories, Hercules, CA) per the manufacturer's instructions. HMGB1 was measured in cell culture supernatants using an ELISA kit per the manufacturer's instructions (IBL International, Hamburg, Germany).

Filiberto A.C., Ladd Z., Leroy V., Su G., Elder C.T., Pruitt E.Y., Hensley S.E., Lu G., Hartman J.B., Zarrinpar A., Sharma A.K, & Upchurch GR J.r. (2022). Resolution of inflammation via RvD1/FPR2 signaling mitigates Nox2 activation and ferroptosis of macrophages in experimental abdominal aortic aneurysms. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36(11).

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