HEK293A (293A) cell line was purchased from Thermo Fisher Scientific. HEK293T (293T), HeLa S3, and MCF10A cell lines were purchased from the American Type Culture Collection. HEK293A (293A), HEK293T (293T), and HeLa S3 were cultured in Dulbecco modified Eagle medium supplemented with 10% fetal calf serum. MCF10A cells were cultured in DMEM/F-12 medium with 5% horse serum, 20 ng/ml human EGF, 0.5 μg/ml hydrocortisone, 100 ng/ml cholera toxin, and 10 μg/ml recombinant human insulin.
Hek293a 293a cell line
Manufactured by Thermo Fisher Scientific
The HEK293A (293A) cell line is a widely used human embryonic kidney cell line. It is a common model system for various cell biology studies and applications, including gene expression, protein production, and viral vector development.
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2 protocols using hek293a 293a cell line
1
Cell Culture Protocols for HEK293, HeLa, and MCF10A
2
Cell Culture Protocols for HEK293, HeLa, and MCF10A
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