Acquity ultra high performance liquid chromatograph

Manufactured by Thermo Fisher Scientific

The ACQUITY Ultra High Performance Liquid Chromatograph is a laboratory instrument used for the separation, identification, and quantification of chemical compounds in a liquid sample. It employs high-pressure liquid chromatography technology to achieve efficient and rapid separation of complex mixtures.

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2 protocols using acquity ultra high performance liquid chromatograph

Simulated Gastric and Intestinal Fluid Analysis

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Waters ACQUITY Ultra High Performance Liquid Chromatograph coupled with Thermo Fisher-Exactive Obitrap high resolution mass spectrometer (HRMS) was used for simulated gastric juice and simulated intestinal fluid analysis. DF-101 SA-H collecting heating at constant temperature magnetic stirrer (Nanjing Kohl Instruments and Equipment Co. Ltd., China), 80–2 Electric Centrifuge (Changzhou Guoyu Instruments Manufacturing Co. Ltd., China).
UPLC conditions: Waters ACQUITY UPLC with Wakopak ultra C18-3 (3.0 mm × 250 mm) column and PDA detector.
HRMS conditions: ESI ionization source; automatic sampler mobile phase ratio, acetonitrile:0.1% aqueous formate solution (V:V) = 60:40; flow rate, 200 μL/min; capillary temperature, 250 °C; capillary tube voltage, 60 V; tube voltage, 120 V; injection volume, 0.1 μL; auxiliary gas, 10 L/min; sheath gas, 40L/min; skimmer voltage, 22 V; sketch time, 2 min; scan range/(m/z), 100–1,000, electronic transmission tube temperature, 275 °C; scanning mode, positive and negative ions scanning mode respectively.

Cheng X., Zhang Q., Li Z., Dong C., Jiang S., Sun Y.A, & Wang G. (2020). Determination of behavior of catalpol hexapropionate in simulated gastric conditions by UPLC–ESI–HRMS. Scientific Reports, 10, 11185.

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Metabolomics Analysis Using High-Resolution Mass Spectrometry

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Metabolomics was conducted in partnership with Metabolon’s proprietary analysis as previously described.^{14 (link)} All methods used a Waters Acquity ultrahigh performance liquid chromatograph and a Thermo Fisher Scientific Q-Exactive high-resolution/accurate mass spectrometer interfaced with a heated electrospray ionization (HESI-II) source and Orbitrap mass analyzer operated at 35,000 mass resolution. Raw data files are archived, extracted, and analyzed as described in the Supplementary Methods. Data are available on Metabolomics workbench (ID 3079).

Ramos G.P., Bamidele A.O., Klatt E.E., Sagstetter M.R., Kurdi A.T., Hamdan F.H., Kosinsky R.L., Gaballa J.M., Nair A., Sun Z., Dasari S., Lanza I.R., Rozeveld C.N., Schott M.B., Urrutia G., Westphal M.S., Clarkson B.D., Howe C.L., Marietta E.V., Luckey D.H., Murray J.A., Gonzalez M., Braga Neto M.B., Gibbons H.R., Smyrk T.C., Johnsen S., Lomberk G, & Faubion W.A. (2022). G9a Modulates Lipid Metabolism in CD4 T Cells to Regulate Intestinal Inflammation. Gastroenterology, 164(2), 256-271.e10.

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