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Mir 335 5p

Manufactured by GenePharma
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Sourced in China

MiR-335-5p is a synthetic microRNA (miRNA) product offered by GenePharma. MiR-335-5p is a short, single-stranded RNA molecule that regulates gene expression. It functions as a key regulator of various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Mir 410 3p, by GenePharma (2 mentions) Mir mock, by GenePharma (2 mentions) Mir 410 5p, by GenePharma (2 mentions) Mir 590 5p, by GenePharma (2 mentions) Anti mir 590 5p, by GenePharma (2 mentions) Anti mir 410 5p, by GenePharma (2 mentions) Mmu mir 1896 3p, by GenePharma (2 mentions) Pmirglo mapk10 wt, by GenePharma (2 mentions) Pmirglo mapk10 mut, by GenePharma (2 mentions) Wild type and mutant mapk10, by Genechem (2 mentions) Mutant mapk10, by Genechem (2 mentions)

Close spelling variants of this product

MiR-335-5p inhibitor MiR-335-5p mimics

4 protocols using mir 335 5p

1

Cell Cycle Analysis and Luciferase Assay

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Transfected cells in the logarithmic growth phase were inoculated onto a 6-well plate and cultured for 1 day. Cells were xed in 70% ethanol for 24 h and treated with propidium iodide and RNase provided with the kit. The cell cycle distribution was detected by ow cytometry.
Dual Luciferase Reporter Assay HEK-293 cells were divided into the miR-30a-3p and pmirGLO empty vectors, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p, and pmirGLO-MAPK10-MuT (GenePharma) co-transfection groups, respectively. Untreated cells were used as controls. Wild-type and mutant MAPK10 were synthesized by GeneChem (Shanghai, China) as follows: wild-type MAPK10, up 5 -cATTTAACTTCTAGTTGCTCTTGCc-3 and down 5 -tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3 ; and mutant MAPK10, up 5 -cATTTAACTTCTAGTTGATATCGCc-3 and down 5 -tcgagGCGATATCAACTAGAAGTTAAATgagct-3 . Cells were inoculated onto 96-well plates and cultured for 24 h. Luciferase activity was detected using a microplate reader. Renilla luciferase was used as an internal reference.
Gao Y., Wang Y., Wang X., Zhao C., Wang F., Du J., Zhang H., Shi H., Feng Y., Li D., Yan J., Yao Y., Hu W., Ding R., Zhang J., Wang L., Huang C., & Zhang J. (2020). miR-335-5p suppresses gastric cancer progression by targeting MAPK10.
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2

Dual Luciferase Assay for miRNA-MAPK10 Interaction

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Transfected cells in the logarithmic growth phase were inoculated onto a 6-well plate and cultured for 1 day. Cells were xed in 70% ethanol for 24 h and treated with propidium iodide and RNase provided with the kit. The cell cycle distribution was detected by ow cytometry.
Dual Luciferase Reporter Assay HEK-293 cells were divided into the miR-335-5p and pmirGLO empty vectors, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p, and pmirGLO-MAPK10-MuT (GenePharma) co-transfection groups, respectively. Untreated cells were used as controls. Wild-type and mutant MAPK10 were synthesized by GeneChem (Shanghai, China) as follows: wild-type MAPK10, up 5 -cATTTAACTTCTAGTTGCTCTTGCc-3 and down 5 -tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3 ; and mutant MAPK10, up 5 -cATTTAACTTCTAGTTGATATCGCc-3 and down 5 -tcgagGCGATATCAACTAGAAGTTAAATgagct-3 . Cells were inoculated onto 96-well plates and cultured for 24 h. Luciferase activity was detected using a microplate reader. Renilla luciferase was used as an internal reference.
Gao Y., Wang Y., Wang X., Zhao C., Wang F., Du J., Zhang H., Shi H., Feng Y., Li D., Yan J., Yao Y., Hu W., Ding R., Zhang J., Wang L., Huang C., & Zhang J. (2020). miR-335-5p suppresses gastric cancer progression by targeting MAPK10.
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3

Analyzing miRNA Interactions via FRET

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MiR-mock, miR-410-3p, miR-410-5p, miR-590-5p, anti-miR-590-5p, anti-miR-410-5p, mmu-miR-1896-3p, and miR-335-5p were purchased from Gene Pharma (Suzhou, China). Sequence information for the miRNAs was obtained from the miRBase 19 (link)-23 (link)(http://microrna.sanger.ac.uk/sequences/). MiR-mock, miR-590-5p, and miR-410-5p were labeled with FAM at 5' end. mmu-miR-1896-3p, miR-335-5p, anti-miR-590-5p, anti-miR-410-5p, and miR-410-3p were labeled with Cy3 at 3' end. MiR-mockFAM + miR-410-3pCy3, miR-410-5pFAM + miR-410-3pCy3, and miR-410-5pFAM + mmu-miR-1896-3p, and miR-335-5pCy3 were co-transfected into RWPE-1 cells. miR-410-5pFAM + anti-miR-410-5pCy3, and miR-590-5pFAM + anti-miR-590-5pCy3 were co-transfected into RWPE-1 cells, as a positive control. The samples were analyzed at the indicated time points using the Amnis Image Stream mk II with the excitation wavelength set to 488 nm (FAM excitation) and emission set to 516 nm for FAM emission, and 568 nm for Cy3 emission. When the oligonucleotides bound to the complementary sequences in cells, a FRET signal was observed (downregulation of the FAM emission intensity and the detection of Cy3 fluorescence). All oligonucleotides are listed in Table S1.
Wang J., Ye H., Zhang D., Cheng K., Hu Y., Yu X., Lu L., Hu J., Zuo C., Qian B., Yu Y., Liu S., Liu G., Mao C, & Liu S. (2017). Cancer-derived Circulating MicroRNAs Promote Tumor Angiogenesis by Entering Dendritic Cells to Degrade Highly Complementary MicroRNAs. Theranostics, 7(6), 1407-1421.
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4

Fluorescent miRNA Binding Kinetics

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MiR-mock, miR-410-3p, miR-410-5p, miR-590-5p, anti-miR-590-5p, anti-miR-410-5p, mmu-miR-1896-3p, and miR-335-5p were purchased from Gene Pharma (Suzhou, China). mmu-miR-1896-3p, miR-335-5p, miR-410-3p, miR-590-5p, and miR-410-5p were 5'-labeled with FAM and 3'-labeled with Cy3 (referred to as mmu-miR-1896-3p Fluor, miR-335-5pFluor, miR-410-3pFluor, miR-590-5pFlour, and miR-410-5pFlour respectively. MiR-mock + miR-410-3pFluor, miR-410-5p + miR-410-3pFluor, miR-410-5p + mmu-miR-1896-3p, and miR-410-5p + miR-335-5pFluor were co-transfected into RWPE-1 cells. miR-410-5p Fluor + anti-miR-410-5p, and miR-590-5p Fluor + anti-miR-590-5p were co-transfected into RWPE-1 cells as a positive control. The samples were also analyzed on the Amnis Image Stream mkII with the excitation wavelength set to 488 nm (FAM excitation) at the indicated time points; the emission wavelength was set to 516 nm (FAM emission) and 568 nm (Cy3 emission). When the labeled miRNA was degraded after binding to another oligonucleotide, Cy3 fluorescence was suppressed and the FRET signal vanished (downregulation of Cy3 emission intensity and upregulation of FAM fluorescence intensity). All oligonucleotides are listed in Table S1.
Wang J., Ye H., Zhang D., Cheng K., Hu Y., Yu X., Lu L., Hu J., Zuo C., Qian B., Yu Y., Liu S., Liu G., Mao C, & Liu S. (2017). Cancer-derived Circulating MicroRNAs Promote Tumor Angiogenesis by Entering Dendritic Cells to Degrade Highly Complementary MicroRNAs. Theranostics, 7(6), 1407-1421.
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