CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections, mice were anesthetized under ice for 4 min, then visualized by Vevo 770 ultrasound backscatter microscopy (VisualSonics) using Aquasonic 100 ultrasound gel (Parker Laboratories). Four slow-pulse injections of 60 nl of CTB-555 (2 mg/ml) were deposited on each side of the midline at L1–L2 using a pulled glass micropipette with a nanojector (Nanoject II, Drummond Scientific) to obtain bilateral labeling. The mice were placed on a heating pad for recovery. Mice were euthanized at P7, allowing the retrograde tracers 2 d for transport.
Ctb 555 recombinant retrograde tracer
Manufactured by Thermo Fisher Scientific
The CTB-555 is a recombinant retrograde tracer produced by Thermo Fisher Scientific. Its core function is to facilitate the tracing of neuronal connections by retrograde labeling of neurons.
Automatically generated - may contain errors
2 protocols using ctb 555 recombinant retrograde tracer
1
Retrograde Labeling of Lumbar Segments
2
Retrograde Labeling of Lumbar Spinal Segments
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CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections, mice were anesthetized under ice for 4 minutes, then visualized by Vevo 770 ultrasound backscatter microscopy (VisualSonics, Toronto, Canada) using Aquasonic 100 ultrasound gel (Parker Laboratories, Fairfield, NJ). 4 slow-pulse injections of 60 nl of CTB-555 (2 mg/ml) were deposited on each side of the midline at L1-L2 using a pulled glass micropipette with a nanojector (Nanoject II, Drummond Scientific, Broomall, PA) to obtain bilateral labeling. The mice were placed on a heating pad for recovery. Mice were euthanized at P7, allowing the retrograde tracers 2 days for transport.
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