Oddessy imaging system

Manufactured by LI COR

Sourced in United States

The Li-Cor Odyssey Imaging System is a versatile fluorescence-based imaging platform designed for a wide range of applications in life science research. The system is capable of simultaneously detecting and quantifying multiple fluorescent signals on a single membrane or gel-based sample.

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Lab products found in correlation

Western lightning plus ecl enhanced chemiluminescence substrate, by PerkinElmer (1 mentions)

Close spelling variants of this product

Oddessy fluorescent imaging system Oddessy system

3 protocols using oddessy imaging system

Western Blotting Protocol

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Western blotting was performed using standard protocols. Primary antibodies used for western blotting are listed in Table S1. Secondary antibodies were goat anti-rabbit and goat anti-mouse immunoglobulin G-horseradish peroxidase-conjugated (BioRad, 1:10,000 dilution). Membranes were developed using Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer) and visualized using photographic film. Alternatively, IRDye (Li-COR Biosciences, Lincoln, NE, USA) secondary antibodies were used for visualization with the Li-Cor Oddessy Imaging System (Li-COR Biosciences, Lincoln, NE, USA).

Boulay G., Sandoval G.J., Riggi N., Iyer S., Buisson R., Naigles B., Awad M.E., Rengarajan S., Volorio A., McBride M.J., Broye L.C., Zou L., Stamenkovic I., Kadoch C, & Rivera M.N. (2017). Cancer-Specific Retargeting of BAF Complexes by a Prion-like Domain. Cell, 171(1), 163-178.e19.

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Antibody-based Nuclear Protein Isolation

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Nuclear extracts were prepared to a final concentration of 2.5 mg/ml with IP buffer 2. For each IP, 75ug (30 μL) of nuclear extract was incubated with 2.5 μg of antibody overnight at 4°C and then for 1h with 15 μL pre-washed Protein G Sepharose beads. After centri-fugation (10,000 rpm for 1min) 45 μL of the supernatant was either saved or used for another round of IP. In total 3 rounds of IP were performed. Quantitative densitometry analyses were performed with the Li-Cor Oddessy Imaging System (Li-COR Biosciences, Lincoln, NE, USA).

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Nuclear Protein Immunoprecipitation and Quantification

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Nuclear extracts were prepared to a final concentration of 2.5 mg/ml with IP buffer 2. For each IP, 75ug (30 mL) of nuclear extract was incubated with 2.5 mg of antibody overnight at 4°C and then for 1h with 15 mL pre-washed Protein G Sepharose beads. After centrifugation (10,000 rpm for 1min) 45 mL of the supernatant was either saved or used for another round of IP. In total 2–3 rounds of IP were performed. Quantitative densitometry analyses were performed with the Li-Cor Oddessy Imaging System (Li-COR Biosciences, Lincoln, NE, USA).

Sandoval G.J., Pulice J.L., Pakula H., Schenone M.A., Takeda D.Y., Pop M., Boulay G., Williamson K.E., McBride M.J., Pan J., Pierre R.S., Hartman E., Garraway L.A., Carr S.A., Rivera M.N., Li Z., Ronco L., Hahn W.C, & Kadoch C. (2018). Binding of TMPRSS2-ERG to BAF chromatin remodeling complexes mediates prostate oncogenesis. Molecular cell, 71(4), 554-566.e7.

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