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2 protocols using butyl lactate

1

Formulation and Evaluation of CZL Delivery

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CZL was kindly supplied by Pharco Pharmaceutical Inc (Alexandria, Egypt). Polyglycolized glycerides (Maisine® 35-1, Labrafil® M 1944 CS, Transcutol® P, Capryol® 90 [CP], Lauroglycol® 90 [LG], Labrafac®, and Solubilisant Gamma® 2429 [SG]) were kindly donated by Gattefossé (Nanterre, France). Butyl lactate (BL), Triacetin, Ethyl oleate, and Ethyl caprylate were purchased from Sigma-Aldrich Co. (St Louis, MO, USA) and Miglyol® 812 from Sasol (Hamburg, Germany). Aerosil® 200 and Aeroperl® 300 (amorphous and granular silicon dioxide) were from Degussa (Marl, Germany). Acetonitrile high performance liquid chromatography (HPLC) grade from Romil Ltd (Cambridge, England). Avicel PH 101 (microcrystalline cellulose) from JRS (Rosenberg, Germany). Absolute ethanol (propanol) from Merck KGaA (Darmstadt, Germany). Propylene glycol from Al Gomhuria Medicine Trade Co. (Cairo, Egypt). All other chemicals used were of analytical grade.
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2

Nanoparticle Formulation Development

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Trilaurin (TL), tristearin, myristic acid, glyceryl monostearate, ethyl acetate (EA), benzyl alcohol (BenzOH), triacetin (TA), butyl lactate (BL), polysorbate (20, 40, 80), sodium taurodeoxycholate (Na TdC), sodium taurocholate (Na TC), sodium glycocholate (Na GC), sodium cholate (Na C), Pluronic® F68, polyvinyl alcohol (PVA) 9000, PVA 14000, 1,2 propanediol, curcumin and Sepharose® CL 4B were purchased from Sigma (Dorset, UK). Trimyristin was from Alfa Aesar (Karlsruhe, Germany), glyceryl dibehenate was from Gattefossé (Weil am Rhein, Germany), Epikuron® 200 (lecithin-phosphatydil coline 92%) from Cargill (Minneapolis, MN, USA), and Cremophor® RH 60 (PEG-60 hydrogenated castor oil) from BASF (Ludwigshafen, Germany).
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