Peptides for LCMV antigens GP33 (KAVYNFATM), GP276 (SGVENPGGYCL), NP396 (FQPQNGQFI), and GP61 (GLNGPDIYKGVYQFKSVEFD) were purchased from AnaSpec. PE-conjugated H2Db GP33-KAVYNFATM and I-Ab LCMV GP66-77 tetramers were provided by the NIH Tetramer Facility (Atlanta, GA).
Glngpdiykgvyqfksvefd
Manufactured by AnaSpec
Description not available
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using glngpdiykgvyqfksvefd
1
LCMV Antigen Peptides for Tetramer Assays
2
Ex Vivo Splenocyte Stimulation
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Two million splenocytes were plated in 96-well round bottom plates in RPMI complete media (RPMI; 10% FBS, 1% penicillin/streptomycin, 1% L-glutamine, 1% HEPES, 1% nonessential amino acids, 1% sodium pyruvate, 50 μM 2-mercaptoethanol, 1 μg/ml of Brefeldin A) with 2 μg/ml GP33-41 peptide (KAVYNFATC; Anaspec) [37 (link)] or 4 μg/ml GP61-80 peptide (GLNGPDIYKGVYQFKSVEFD; Anaspec) [38 (link)] at 37°C for five hours.
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