FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2), AF647-conjugated anti-mouse CD49a (clone Ha31/8) antibodies, and purified anti-mouse CD16/32 (Fc Block™) were purchased from BD Pharmingen (San Diego, CA). APC-conjugated anti-mouse α4β7 (clone DATK32) was purchased from eBioscience (San Diego, CA). The H-2Db-restricted HPV16 E7aa49-57 peptide, RAHYNIVTF, was synthesized by Macromolecular Resources (Denver, CO) at a purity of ≥ 80%. PE-conjugated, HPV16 E7aa49-57 peptide loaded H-2Db tetramer was provided by NIH Tetramer Core Facility and has been used in our previous studies17 (link), 19 (link).
Apc conjugated anti mouse α4β7 clone datk32
Manufactured by Thermo Fisher Scientific
Sourced in United States
The APC-conjugated anti-mouse α4β7 (clone DATK32) is a flow cytometry reagent that can be used to detect and analyze the expression of the α4β7 integrin on mouse cells. The α4β7 integrin is a cell surface receptor that plays a role in lymphocyte homing and trafficking. The APC (Allophycocyanin) fluorescent dye is conjugated to the anti-mouse α4β7 antibody, allowing for the detection of α4β7-positive cells by flow cytometry.
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Lab products found in correlation
2 protocols using apc conjugated anti mouse α4β7 clone datk32
1
Phenotypic Analysis of Antigen-Specific T Cells
2
Multiplex Detection of Tumor-Specific T Cells
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FITC and PE-conjugated anti-mouse CD8a (clone 53.6.7), FITC-conjugated anti-mouse IFN-γ (clone XMG1.2), AF647-conjugated anti-mouse CD49a (clone Ha31/8) antibodies, and purified anti-mouse CD16/32 (Fc Block) were purchased from BD Pharmingen (San Diego, CA, USA). APC-conjugated anti-mouse α4β7 (clone DATK32) was purchased from eBioscience (San Diego, CA, USA). The H-2Db-restricted HPV16 E7aa49-57 peptide, RAHYNIVTF, was synthesized by Macromolecular Resources (Denver, CO, USA) at a purity of⩾80%. PE-conjugated, HPV16 E7aa49-57 peptide-loaded H-2Db tetramer was provided by NIH Tetramer Core Facility and has been used in our previous studies.17 (link), 19 (link)
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