Paclitaxel ptx

Paclitaxel (PTX) was purchased from Bristol-Myers Squibb, dissolved in Ethanol to make 7 μM stock concentration. JNK-IN-8 (Calbiochem, UK), with a working concentration of 10 µM was prepared in DMSO. MDR1/ABCB1, E-cadherin, N-cadherin, Vimentin, Snail, TCF8/ZEB1, β-catenin, Axin-1, Claudin, PI3K p110γ, p-PDK-1, Akt, p-Akt, p-c-Raf, Cyclin D1, p-GSK3β, p-p38 MAPK, p38 MAPK, p-p44/42, c-jun, p- SAPK/JNK, SAPK/JNK, p-AMPK, Wnt3a, Wnt5a/b, p-LRP6, LRP6, Dvl2, Dvl3, β-actin, GAPDH primary, and secondary antibodies were used at 1:1000 dilutions and were from Cell Signaling Technology (CST, Beverly, MA, USA).

Cell culture reagents (DMEM and fetal bovine serum) were from Invitrogen/Gibco. [U-14C]-glucose were from Shenzhen Zhonghe Headway Bio-Sci & Tech Co. Anti-Flag M2 (Sigma- Aldrich, St Louis, MO, USA), monoclonal anti-HA (Covance, Dedham, MA, USA), anti-SREBP-1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-β-actin (Cell Signaling Technology, Danvers, MA, USA) anti-EGFR (Cell Signaling Technology) and anti–pi-EGFR (Cell Signaling Technology) antibodies were purchased. Oleic acid, stearic acid and arachidonic acid were purchased from Sigma-Aldrich. Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) was used in transient transfection according to manufacturer's protocol. Paclitaxel (PTX) was from Bristol-Myers Squibb. Cisplatin and gefitinib were obtained from Selleck Chemicals.