Polycarbonate etched membrane

Manufactured by Cytiva

Sourced in United States

Polycarbonate-etched membrane is a type of filtration membrane made from polycarbonate material. The membrane is etched using a chemical process to create a defined pore structure. This membrane is designed for various laboratory applications that require precise filtration.

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Lab products found in correlation

Dulbecco s phosphate buffer saline pbs, by Merck Group (2 mentions) Infinite 200 pro multimode reader, by Tecan (1 mentions) Uv star 96 well microplate, by Greiner (1 mentions) Collagenase type 1, by Merck Group (1 mentions) Zetasizer nano zsp, by Malvern Panalytical (1 mentions) Cholesterol, by Merck Group (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine dmpc, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

Whatman Nuclepore track-etched polycarbonate membranes Nuclepore track-etched polycarbonate membrane filter Nuclepore Track-Etched polycarbonate membranes Polycarbonate track-etched membrane Polycarbonate track-etched membrane filters Whatman Nucleopore track-etched polycarbonate membrane Polycarbonate membranes

2 protocols using polycarbonate etched membrane

Liposome Preparation and Optical Analysis

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Liposomes were prepared using the ethanol injection method. Lipids were weighed and dissolved in absolute ethanol and subsequently injected into calcium-free Dulbecco’s phosphate buffer saline (PBS; Sigma-Aldrich) preheated to 65 °C reaching a final lipid concentration of 50 mM. The liposomes were extruded five times using a high-pressure Lipex extruder (Northern Lipids, Canada) through 400, 200, and 100 nm polycarbonate-etched membrane (Whatman, Newton, MA, USA) at 65 °C.
For the absorbance measurements, liposomes were diluted 50-fold and measured in a UV-star 96-well microplate (Greiner bio-one, Germany) using the Infinite 200PRO multimode reader (TECAN, Switzerland) controlled with the i-control 1.10 software. Based on the absorbance data, a Lorentzian function was fitted for each of the phospholipids using the curve fitting toolbox in Matlab (version 2021b). The Lorentzian function Eq. (2) is:

L = a / ({(x - b)}^{2 + c}) + d

Where the constants a, b, c, and d denote the Lorentzian amplitude, center, width, and offset respectively, and x denotes the Lorentzian variable - photon energy.

Adir O., Albalak M.R., Abel R., Weiss L.E., Chen G., Gruber A., Staufer O., Kurman Y., Kaminer I., Shklover J., Shainsky-Roitman J., Platzman I., Gepstein L., Shechtman Y., Horwitz B.A, & Schroeder A. (2022). Synthetic cells with self-activating optogenetic proteins communicate with natural cells. Nature Communications, 13, 2328.

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Collagenase-Loaded Liposomal Delivery

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Collagenase type-I (Sigma-Aldrich, St. Louis, MO, USA) was encapsulated in 100-nm liposomes. A lipid mixture of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC; Avanti Polar Lipids, Alabaster, AL, USA), cholesterol (Sigma-Aldrich) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-polyethylene glycol 2000 (PEG-DSPE; Avanti), at three different molar ratios of: 95:0:5, 80:15:0 and 56:39:5, respectively, were dissolved in absolute ethanol. The lipid solution was added to calcium free Dulbecco's Phosphate Buffer Saline (PBS; Sigma-Aldrich) solution containing 2mg/ml collagenase to reach a lipid concentration of 50mM, at 50°C. The liposomes were downsized using a Lipex extruder (Northern Lipids, Vancouver, Canada) five times through each 400, 200 and 100-nm polycarbonate etched membrane (Whatman, Newton, MA, USA) at 40°C with a maximal working pressure of 10bar. The non-encapsulated protein was removed by dialysis using a 1000kDa cutoff membrane (Spectrum Labs, CA, USA) against PBS solution (1:1000 vol ratio); the external PBS was replaced after 1, 3 and 24 hours, at 4°C. Liposomes were sized using a Zetasizer NanoZSP (Malvern Instruments, Worcestershire, UK) using disposable polystyrene cuvettes after diluting the samples 1:100 in PBS.

Zinger A., Koren L., Adir O., Poley M., Alyan M., Yaari Z., Noor N., Krinsky N., Simon A., Gibori H., Krayem M., Mumblat Y., Kasten S., Ofir S., Fridman E., Milman N., Lübtow M.M., Liba L., Shklover J., Shainsky-Roitman J., Binenbaum Y., Hershkovitz D., Gil Z., Dvir T., Luxenhofer R., Satchi-Fainaro R, & Schroeder A. (2019). Collagenase Nanoparticles Enhance the Penetration of Drugs into Pancreatic Tumors. ACS nano, 13(10), 11008-11021.

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