Lipopolysaccharides (LPS) from Escherichia coli O26:B6, fluorescein isothiocyanate (FITC)-labeled wheat germ agglutinin (WGA) lectin from Triticum vulgaris, and tetramethylrhodamine (TMR)-labeled dextran (average molecular weight, 75 kDa [TMR-dex75]) were purchased from Sigma-Aldrich Co. (St Louis, MO). The mouse-soluble syndecan-1 (Sdc-1) enzyme-linked immunosorbent assay (ELISA) kit from Diaclone SAS (Besancon Cedex, France) was purchased. Ketamine hydrochloride, xylazine hydrochloride, and rhodamine 6G were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Sevoflurane was purchased from Pfizer Japan Inc. (Tokyo, Japan), and used for anesthesia using a small animal anesthetizer (MK-A110D, Muromachi Kikai Co., Ltd., Tokyo, Japan).
Ketamine hydrochloride
Manufactured by Fujifilm
Sourced in Japan
Description not available
Automatically generated - may contain errors
2 protocols using ketamine hydrochloride
1
Visualizing Syndecan-1 in Endotoxemia
2
Anesthesia Protocol for Rat Studies
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Male Lewis rats weighing 200–250 g (Sankyo Labo Service, Tokyo, Japan) were used in the study. All experiments were conducted in accordance with the Animal Care Committee of the Jikei University School of Medicine and the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research. Rats were anesthetized with a mixture (1:1) of ketamine hydrochloride (10 mg/kg; Wako Pure Chemicals Industries, Osaka, Japan) and xylazine hydrochloride (4 mg/kg; Wako).
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