Alexa fluor 488

Stevioside (STE) (purity ≥98%) was obtained from MedChemExpress (Shanghai, China). Recombinant mouse IL-1β was acquired from Novoprotein (China). Safranin-O/Fast Green and collagenase-II were procured from Solarbio (Beijing, China). Antibodies of collagen II, aggrecan, Nrf2, ADAMTS-4, and Lamin B1 were purchased from Abcam (Cambridge, UK). GAPDH, p65, HO-1, IkBα, and iNOS were purchased from Boster Biological Technology (Wuhan, China). MMP-13 and COX-2 were obtained from Cell Signaling Technology (Danvers, MA, USA). Fetal bovine serum (FBS), Dulbecco's modified Eagles' medium (DMEM)/F12, and 0.25% trypsin-ethylenediaminetetraacetic acid (trypsin–EDTA) were acquired from Gibco (Grand Island, NY, USA). Bovine serum albumin (BSA) was procured from Beyotime Biotechnology (Shanghai, China). The second antibodies of Goat Anti-Mouse IgG, Goat Anti-Rabbit IgG, Alexa Fluor®594 and Alexa Fluor®488 labeled were purchased from Bioworld (OH, USA).

Anisomycin, Collagenase-II, Safranin-O/Fast Green, and dimethylsulfoxide (DMSO) were obtained from Solarbio Science & Technology Co., Ltd. (Beijing, China). Primary antibodies of Collagenase II, Aggrecan, ADAMTS-5, and MMP-13 were gained from Abcam (Cambridge, MA, USA). Primary antibodies directed against P-JNK and JNK were procured from Cell Signaling Technology (MA, USA). Primary antibodies against Bcl-2 and GAPDH were procured from ProteinTech Group (Wuhan, China). Primary antibodies against cleaved caspase-3 and Bax were procured from Affinity Biosciences (Cincinnati, OH, USA). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM)/F12 were obtained from Gibco (Grand Island, NY, USA). Tert-Butyl hydroperoxide solution (TBHP) was procured from Sigma-Aldrich (St Louis, MO, USA). The secondary antibodies of Goat Anti-Mouse IgG, Goat Anti-Rabbit IgG, Alexa Fluor^®488 and labeled Alexa Fluor^®594 were obtained from bioWORLD (OH, USA). TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). The BCA protein assay kit was procured from Beyotime Biotechnology (Shanghai, China). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Laboratories (Kumamoto, Japan).The In Situ Cell Death Detection Kit was purchased from Roche (San Francisco, CA, USA). Caspase-3 colorimetric assay kit was obtained from Keygen Biotech Co., Ltd. (Nanjing, China).