Log In Sign up
The largest database of trusted experimental protocols

Antibody to hif 1α

Manufactured by Novus Biologicals
Visit Supplier

The Antibody to HIF-1α is a laboratory reagent designed to detect and quantify the presence of the Hypoxia-Inducible Factor 1-alpha (HIF-1α) protein in biological samples. HIF-1α is a transcription factor that plays a crucial role in cellular response to low oxygen conditions.

Automatically generated - may contain errors

Lab products found in correlation

Bip grp78, by Cell Signaling Technology (1 mentions) Phospho eif2α, by Santa Cruz Biotechnology (1 mentions) Antibody to β actin, by Merck Group (1 mentions) Phospho 4ebp1, by Cell Signaling Technology (1 mentions) Atp assay kit, by Promega (1 mentions) Phospho ampk, by Cell Signaling Technology (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) Phospho p70s6k, by Cell Signaling Technology (1 mentions) Glycyrrhizin, by Merck Group (1 mentions) Oligomycin, by Merck Group (1 mentions) Axin1, by Cell Signaling Technology (1 mentions) Notch2, by Cell Signaling Technology (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions) Notch1, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Verteporfin, by Merck Group (1 mentions) Hdac2, by Cell Signaling Technology (1 mentions) Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions) Hmgb1, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

2 protocols using antibody to hif 1α

1

Antibody Reagents for Cellular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody to p62 (# 610833) was purchased from BD Biosciences (Franklin Lakes, NJ, USA). Antibody to BNIP3 (# 10433) and COX (subunit 1; # 90668) were from Abcam (Cambridge, MA, USA). Antibody to β-actin (# A5316) was from Sigma-Aldrich (St Louis, MO, USA). Antibodies to 4EBP1 (#9452), phospho-4EBP1 (# 9459), p70-S6K (# 9202), phospho-p70-S6K (# 9205), AMPK (# 2532), phospho-AMPK (# 4188), calnexin (#C5C9), COX (subunit 4) (#4844), VDAC (# 4661), Bip/Grp78 (# 3177) and LC3 (# 2775) were from Cell Signaling (Danvers, MA, USA). Antibody to p27/Kip1 (# 610241) was from BD Transduction Laboratories (San Jose, CA). Antibody to HIF-1α was from Novus Biologicals (# NB100-105). Antibody to eIF2-α (# sc-11386) and phospho-eIF2-α (# sc-12412) were from Santa Cruz Biotechnology (Santa Cruz, CA). The kit for pimonidazole staining was from NPI Inc. (Belmont, MA). ATP assay kit was from Promega (Madison, WI, USA). The DiverseSet (10,000 compounds) used for screening was purchased from ChemBridge. The original ChemBridge compound 5625138 was resynthesized in gram scale by OncoTargting AB (Uppsala, Sweden). The resynthesized compound generated identical results as the original compound. All compounds were dissolved in dimethylsulphoxide (DMSO). A final concentration of 0.5% DMSO was reached in cell cultures; control wells received solvent only (that is, final 0.5% DMSO concentration).
Zhang X., Fryknäs M., Hernlund E., Fayad W., De Milito A., Olofsson M.H., Gogvadze V., Dang L., Påhlman S., Schughart L.A., Rickardson L., D′Arcy P., Gullbo J., Nygren P., Larsson R, & Linder S. (2014). Induction of mitochondrial dysfunction as a strategy for targeting tumour cells in metabolically compromised microenvironments. Nature Communications, 5, 3295.
+ Open protocol
+ Expand
2

Investigating Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies to HMGB1 (#3935), YAP (#14074), TAZ (#4883), Axin1 (#2087), β-catenin (#9587), Notch1 (#3608), Notch2 (#5732), HDAC2 (#5113), PCNA (#2586), cyclin D1 (#2922), cleaved-caspase 3 (#9661), γH2AX (#9718), GAPDH (#2118), and actin (#3700) were obtained from Cell Signaling Technology. The antibody to GABPα (#21542-1-AP) was obtained from Proteintech Group. The antibody to p73 (#ab26123) was obtained from Abcam. The antibody to HIF1α (#NB100-105) was obtained from NOVUS. Diethylnitrosamine (#N0258), glycyrrhizin (#CDS020796), verteporfin (#SML0534), glucose (#G8270), oligomycin (#O4876), and 2-deoxy-D-glucose (“2-DG”, #D8375) were obtained from Sigma.
Chen R., Zhu S., Fan X.G., Wang H., Lotze M.T., Zeh HJ I.I.I., Billiar T.R., Kang R, & Tang D. (2018). HMGB1 Controls Liver Cancer Initiation through YAP-dependent Aerobic Glycolysis. Hepatology (Baltimore, Md.), 67(5), 1823-1841.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!