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B6 fvb cg

Manufactured by Jackson ImmunoResearch
Sourced in United States

B6.FVB(Cg) is a mouse strain that serves as a source of biological materials for research purposes. It is a congenic strain, meaning it has been genetically modified to have specific genetic characteristics.

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4 protocols using b6 fvb cg

1

Genotyping and Maintenance of Mouse Strains

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Mice were maintained and used according to guidelines for animal care of the NIH and the University Committee on Animal Resources at the University of Rochester. Mice of the following strains C57BL/6J (Jackson Labs, Bar Harbor, ME, USA; stock number 664); Rpl22tm1.1Psam on a C57BL/6J background (Jackson Labs; stock number 011029)138 (link); Mov10l1tm1.1Jw on a mixed 129×1/SvJ × C57BL/6J background11 (link); and Tg(Neurog3-cre)C1Able/J on a B6.FVB(Cg) background (Jackson Labs; stock number 006333)139 (link) were genotyped as described. Comparisons of compound mutants and controls involving Mov10l1 CKO mutation were performed using siblings from individual litters. White Leghorn testes of the Cornell Special C strain from 1-year-old roosters were used according to guidelines for animal care of the NIH and the University Committee on Animal Resources at the University of Rochester.
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2

Characterizing Animal Models for Germline Development

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All experiments were reviewed and approved by the University of Rochester’s University Committee on Animal Resources, performed in an PHS Assured and AAALAC, Int. accredited facility, and the study is compliant with all relevant ethical regulations regarding animal research. Mice of the following strains C57BL/6J (Jackson Labs, Bar Harbor, ME, USA; stock number 664); Rpl22tm1.1Psam on a C57BL/6J background (Jackson Labs; stock number 011029)17 (link); Mov10l1tm1.1Jw on a mixed 129X1/SvJ × C57BL/6J background21 (link); Tg(Neurog3-cre)C1Able/J on a B6.FVB(Cg) background (Jackson Labs; stock number 006333)50 (link); and Tg(Ddx4-cre)1Dcas on a 129X1/SvJ background (Jackson Labs; stock number 006954) were genotyped as described51 (link). Comparisons of compound mutants and controls involving Mov10l1 mutation or a RiboTag allele were performed using male siblings from individual litters at adult stage. Male Plndc1mut mice on a C57BL/6J background10 (link) and Tdrd5tm1a mice on a C57BL/6J background32 (link) at adult stage were raised at Michigan State University according to NIH guidelines for animal care. Testes from adult green lizards (Carolina Biological Supply Company, Burlington, NC, USA) were used for Ribo-seq. Araucana strain testes from one-year old roosters were used for polysome gradients.
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3

Characterizing Animal Models for Germline Development

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All experiments were reviewed and approved by the University of Rochester’s University Committee on Animal Resources, performed in an PHS Assured and AAALAC, Int. accredited facility, and the study is compliant with all relevant ethical regulations regarding animal research. Mice of the following strains C57BL/6J (Jackson Labs, Bar Harbor, ME, USA; stock number 664); Rpl22tm1.1Psam on a C57BL/6J background (Jackson Labs; stock number 011029)17 (link); Mov10l1tm1.1Jw on a mixed 129X1/SvJ × C57BL/6J background21 (link); Tg(Neurog3-cre)C1Able/J on a B6.FVB(Cg) background (Jackson Labs; stock number 006333)50 (link); and Tg(Ddx4-cre)1Dcas on a 129X1/SvJ background (Jackson Labs; stock number 006954) were genotyped as described51 (link). Comparisons of compound mutants and controls involving Mov10l1 mutation or a RiboTag allele were performed using male siblings from individual litters at adult stage. Male Plndc1mut mice on a C57BL/6J background10 (link) and Tdrd5tm1a mice on a C57BL/6J background32 (link) at adult stage were raised at Michigan State University according to NIH guidelines for animal care. Testes from adult green lizards (Carolina Biological Supply Company, Burlington, NC, USA) were used for Ribo-seq. Araucana strain testes from one-year old roosters were used for polysome gradients.
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4

Animal Care for Genetic Studies

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Mice were maintained and used according to guidelines for animal care of the NIH and the University Committee on Animal Resources at the University of Rochester. Mice of the following strains C57BL/6J (Jackson Labs, Bar Harbor, ME, USA; stock number 664); Rpl22 tm1.1Psam on a C57BL/6J background (Jackson Labs; stock number 011029) 136 ; Mov10l1 tm1.1Jw on a mixed 129X1/SvJ × C57BL/6J background 11 ; and Tg(Neurog3cre)C1Able/J on a B6.FVB(Cg) background (Jackson Labs; stock number 006333) 137 were genotyped as described. Comparisons of compound mutants and controls involving Mov10l1 CKO mutation were performed using siblings from individual litters. White Leghorn testes of the Cornell Special C strain from one-year old roosters were used according to guidelines for animal care of the NIH and the University Committee on Animal Resources at the University of Rochester.
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