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Cary e 5000

Manufactured by Agilent Technologies
Sourced in United States

The Cary E 5000 is a UV-Vis-NIR spectrophotometer designed for advanced material characterization. It offers a wide measurement range from 175 to 3300 nm and can perform high-resolution absorption, transmission, and reflectance measurements.

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2 protocols using cary e 5000

1

Characterization of Cu@Pt Nanoparticles

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In this work, the UV–VIS absorption spectra of the synthesized Cu@Pt nanoparticles were obtained from a spectrophotometer Cary E 5000 in the range of 300–800 nm using a quartz cell with 10 mm of optical path length (Agilent, USA). FTIR spectra of the samples were measured using Perkin-Elmer Spectrum 1000, in attenuated total reflection mode, and using the spectral range of 4000–380 cm−1. The study also used one instrument in the diffuse reflectance mode at the resolution of 4 cm−1 in KBr pellets (Perkin Elmer, USA). The obtained Cu@Pt nanoparticles were characterized using an Atomic Force Microscope (Agilent, USA). The size and morphology of the synthesized Cu@Pt nanoparticles were characterized using a Transmission Electron Microscope JEOL JEM 1200 EXII, operating at 200 kV. Moreover, we used a Scanning Electron Microscope (HR SEM) Helios NanoLab 660 (FEI). SEM imaging was performed in the immersion mode (Thermo Fisher Scientific, USA).
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2

UV-Vis Characterization of Synthesized AgNPs

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UV-Visible spectroscopy is a broadly used tool to characterize nanoparticles. The aqueous solution of synthesized AgNPs was prepared using distilled water (DW). The solution was sonicated for 5–10 min, and absorbance was measured between 300 and 700 nm using the Cary E 5000 spectrophotometer (Agilent Technologies Inc., Santa Clara, CA, USA), which indicated the synthesis of AgNPs.
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