Phytic acid sodium salt, 3, 5, 6-trichloro-2-pyridinol (TCP) and diethyl thiophosphate (DETP) was purchased from Sigma Chemical Company (St. Louis, MO, USA). Acetonitrile (ACN) of HPLC grade was purchased from Merck. All other chemicals used were of analytical grade. CPyF (Dursban 2E©, 20%), MCP (36%) and MP (50%), harvested green chilli, soybean meal and seeds of green chickpea were purchased from a local market. The seeds were minced in a grinder to obtain green chickpea flour (GrCf).
Phytic acid sodium salt
Manufactured by Merck Group
Sourced in United States
Phytic acid sodium salt is a chemical compound that is commonly used in research and laboratory settings. It functions as a buffer and chelating agent, helping to maintain pH and bind metal ions in various experimental and analytical procedures.
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Lab products found in correlation
3 protocols using phytic acid sodium salt
1
Quantifying Pesticide Residues in Chili Peppers
2
Phytic Acid Purification and Characterization
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Phytic acid sodium salt was purchased from Sigma Chemical, USA. Sephacryl S-200HR was obtained from Amersham Pharmacia Biotech, Sweden. The enzymes and kits for molecular biology experiments were purchased from standard suppliers and used as per manufactures’ instructions. All the general chemicals used for this report were of analytical grade and purchased from either Sisco Research Laboratories or HiMedia Laboratories, Pvt. Ltd, Mumbai, India.
3
Effect of Phytate on Soybean Growth
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The study was performed in a greenhouse trial from May 20th to August 24th, 2019, in a randomized complete block design with 10 blocks including four treatments with two replicates of each treatment. The average temperature during the experiment ranged from 22 to 27°C with a photoperiod of 16/8 h. Detail experimental procedure are available in the supporting information (Methods S1). Briefly, four L plastic pots (7.5″x7.25″) were filled with substrate made from a mixture of sand, turface and sandy loam soil (1:1:1). Seeds of Viking 2518N non-GMO soybean (Glycine max L.) were purchased from Willium Dam Seeds Ltd (ON, Canada). Phytic acid sodium salt (Sigma-Aldrich, ON, Canada) was used as a source of phytate and the dose of phytate (3.3 mg/pot) was adjusted according to previous studies [51, 52] . Microbial suspension was prepared from soil collected from an age-old maple forest in the Gault Nature Reserve at Mont Saint-Hilaire, in Quebec (Fig. S1). Soybean shoots, roots and rhizosphere soil were collected per treatment and immediately transported to the laboratory and measured different parameters. Detail information is available in the supporting information (Methods S2).
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