Plasma glucose concentration was measured using a hexokinase method at a commercial laboratory (Melbourne Pathology, Collingwood, Victoria, Australia) with a CV < 1.9%. Fasting triglycerides were analysed using a Cobas b 101 instrument (Roche Diagnostics Ltd., Basel, Switzerland). Plasma insulin, connecting peptide (C-peptide) and tGLP-1 concentrations were measured using a Luminex Analyzer (MAGPIX®; Human Metabolic Hormone Magnetic Bead Panel, EMD Millipore, Burlington, MA, USA) following manufacturer’s instructions with intra-assay CV’s of 9.8%, 6.8% and 11.4%, respectively.
Magpix human metabolic hormone magnetic bead panel
Manufactured by Merck Group
Sourced in United States
The MAGPIX® Human Metabolic Hormone Magnetic Bead Panel is a lab equipment product developed by Merck Group. It is a multiplex assay system that utilizes magnetic beads to quantify multiple human metabolic hormones simultaneously in a single sample.
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Lab products found in correlation
2 protocols using magpix human metabolic hormone magnetic bead panel
1
Metabolic Biomarker Measurement Protocol
2
Metabolic Biomarker Assay Protocol
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Triglyceride and cholesterol concentrations were analyzed at time of collection from whole blood using a Cobas b 101 instrument (Roche Diagnostics Ltd, Basel, Switzerland). Blood glucose concentrations were determined using an YSI 2900 analyzer (YSI Life Sciences, Yellow Springs, OH, USA). Plasma insulin, connecting peptide (C-peptide), tGLP-1, leptin and TNF-alpha concentrations were determined using a Luminex Analyzer (MAGPIX®; Human Metabolic Hormone Magnetic Bead Panel, EMD Millipore, MA, USA) following manufacturer's instructions and sample preparation. Serum cortisol was determined using an enzyme-linked immunosorbent assay (ELISA; KA0918, Abnova Corporation, Tapei, Taiwan).
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