Phosphate buffer ph 7

SMV was acquired as a generous gift from the Saudi Arabian Japanese (SAJA) Pharmaceutical Company Limited (Jeddah, Saudi Arabia). Coconut oil was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cremophor EL was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Lauroglycol™ FCC was a gift from Gattefosse (Saint-Priest, France). High-performance liquid chromatography–grade methanol and acetonitrile were obtained from Merck (Darmstadt, Germany). Chloroform, absolute ethanol, phosphate buffer pH 7.4, and 0.9% normal saline were purchased from Fisher Scientific UK (Loughborough, Leicestershire, UK). Water was obtained from a Milli-Q water purification system (Millipore, Bedford, MA, USA). All other reagents and chemicals were of analytical grade.

Naftifine was acquired as a generous gift from Pharco Pharmaceutical Company, Egypt. (Alexandria, Egypt). Cetylpyridinium chloride was acquired as a generous gift from Saudi Drugs and Medical Instruments Company (SPIMACO), in (Qassim, Saudi Arabia). Clove oil was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Diethylene glycol monoethyl ether (Transcutol^®) was kindly provided by Gattefossé (Lyon, France) High-performance liquid chromatography grade methanol and acetonitrile were obtained from Merck (Darmstadt, Germany). Chloroform, absolute ethanol, and phosphate buffer pH 7.4 were purchased from Fisher Scientific UK (Loughborough, Leicestershire, UK). All other reagents and chemicals were of analytical grade.