Toltrazuril was purchased from ChemCruz (Santa Cruz, CA, USA). Poly (ε-caprolactone) (PCL, Mw 14 kDa) and sorbitan monostearate were purchased from Sigma-Aldrich (Steinheim, Germany). Eudragit® S100 was obtained from Evonik (Darmstadt, Germany). Polysorbate 80, ethanol, and acetone were purchased from Vetec (Rio de Janeiro, Brazil), and Capric/caprylic triglyceride (CCT) was obtained from Delaware (Porto Alegre, Brazil). Cell culture media and supplements were obtained from BD Difco™ (Franklin Lakes, NJ, USA). All other reagents and solvents used were of analytical or pharmaceutical grade.
Polysorbate 80
Manufactured by Vetec
Sourced in Brazil
Polysorbate 80 is a non-ionic surfactant and emulsifier commonly used in various laboratory applications. It is a viscous, yellowish liquid with a mild odor. Polysorbate 80 is soluble in water and can help improve the solubility and dispersibility of other compounds.
Automatically generated - may contain errors
Lab products found in correlation
Sorbitan monostearate, by Merck Group (1 mentions)
Ethanol, by Vetec (1 mentions)
Eudragit s100, by Evonik (1 mentions)
Acetone, by Vetec (1 mentions)
Protease inhibitor cocktail, by Avantor (1 mentions)
Methanol, by Avantor (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
Normal goat serum (ngs), by R&D Systems (1 mentions)
Thiopental, by Cristália (1 mentions)
Medium chain triglycerides, by Lipoid (1 mentions)
Egg yolk lecithin, by Lipoid (1 mentions)
Acetonitrile, by Tedia (1 mentions)
Ketamine, by Ceva (1 mentions)
Xylazine, by Ceva (1 mentions)
5 aminosalicylic acid, by Merck Group (1 mentions)
3 5 dinitrosalicylic acid, by Merck Group (1 mentions)
Absolute ethanol, by Vetec (1 mentions)
4 protocols using polysorbate 80
1
Toltrazuril-loaded PCL nanoparticles
2
HPLC Analytical Methodology for DEET Formulation
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The active pharmaceutical ingredient N, N-dimethyl-meta-toluamide (DEET) was from Welpharm CO ® . The excipients used to develop the formulation: Stearic Acid -Pharma Nostra ® , Polysorbate 80 and Methyl Paraben -Vetec ® . The purified water used to prepare the mobile phase we obtained through the Sartorius ® system. To develop and validate the HPLC analytical methodology, we used the following reagents: Methanol AG -Merck ® , Hydrochloric Acid ACS and Phosphoric Acid -Sciavicco ® , Sodium Hydroxide ACS -Neon ® , Hydrogen Peroxide -Dynamic ® , Copper Sulfate Pentahydrate -Siavicco ® . The DEET and methyl paraben standard references were from the United States Pharmacopeia Reference Standard. We filtered the mobile phase and diluent through a vacuum filtration system composed of a 0.45 µm membrane. The samples, Active Pharmaceutical Ingredient (API), International Chemical Reference Substances (ICRS) and matrix were filtered through 0.45 µm filters (PTFE -Polytetrafluoroethylene -25 mm -Analítica ® ) and 0.22 µm (PVDF -Polyvinylidene fluoride -25 mm -FilterPro ® ). The chromatographic columns used were C18 125 mm x 4 mm x 5 µm, from ACE ® and Merck ® .
3
Extraction and Characterization of A. satureioides
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A. satureioides was acquired from Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA) da Universidade Estadual de Campinas (São Paulo, Brazil), via a sample deposited in the herbarium with the number 308. Egg yolk lecithin and medium-chain triglycerides were purchased from Lipoid (Ludwigshafen, Germany), polysorbate 80 was acquired from Vetec (Rio de Janeiro, Brazil), and vitamin E was procured from Alpha Química (Cachoeirinha, Brazil). Ultra-fast liquid chromatography (UFLC) required the following reagents: methanol (J.T. Baker, Philipsburg, NJ, USA), acetonitrile (Tedia, Rio de Janeiro, Brazil), and phosphoric acid (Merck, Rio de Janeiro, Brazil). Carbopol® Ultrez 20 was kindly donated by Lubrizol do Brasil Aditivos Ltd.a (São Paulo, Brazil). Albumin for enzyme-linked immunosorbent assay (ELISA) was purchased from Calbiochem (San Diego, CA, USA). Normal goat serum was obtained from R&D Systems (Minneapolis, MN, USA). Protease inhibitor cocktail was purchased from VWR Life Science (Radnor Township, PA, USA). Xylazine and ketamine were purchased from Ceva (Paulínea, Brazil). Ketoprofen was obtained from Merial (Paulínea, Brazil). Finally, thiopental was obtained from Cristália (Itapira, Brazil).
4
Formulation of Starch-Based Biopolymers
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High amylose starch (HAS) (type Hylon VII, 70% amylose and 30% amylopectin) was a gift from National Starch & Chemical (Bridgewater Township, NJ, USA), cellulose nanofibers (CNF) (HD Grade 2000, diameter ≤100 nm, 3.8% w/w dry content from Eucaliptus bleached kraft pulp) were supplied by Suzano Cellulose & Papel (Limeira, Brazil) and pectin (type LM-5206CS—DE < 50%) was provided by CP Kelco (Copenhagen, Denmark). Sodium hydroxide was supplied by Grupo Química (Rio de Janeiro, Brazil), and 37% hydrochloric acid, glucose and potassium phosphate monobasic (98.0–100.5%) were provided by Quimis (Diadema, Brazil). Pancreatin, polysorbate 80 and absolute ethanol were purchased from Vetec (Duque de Caxias, Brazil), and 3,5-dinitrosalicylic acid (DNS) (purity ≥ 98.0%) and 5-aminosalicylic acid (95%) were provided by Sigma-Aldrich Co. (St. Louis, MO, USA).
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