Mia paca 2

Human PDAC cell lines (MIA PaCa-2, PANC-1, HPAF-II, AsPC-1, and Bx PC-3) were obtained from American Type Culture Collection, Manassas, VA, USA. hTERT-HPNE cells were obtained from Dr. Channing Der’s laboratory at UNC, Chapel Hill, NC. MIA PaCa-2 cells were cultured in DMEM (Dulbecco’s Modified Eagle Medium) high-glucose media (GE Healthcare Life Sciences, Chicago, IL, USA) containing 10% (v/v) fetal bovine serum (Atlanta Biologicals, Flowery Branch, GA, USA) and 2.5% (v/v) horse serum (Corning, Corning, NY, USA). PANC-1 cells were cultured in DMEM high-glucose media containing 10% (v/v) FBS. HPAF-II cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (Corning, Corning, NY, USA) containing 10% v/v fetal bovine serum (FBS). AsPC-1 were cultured in RPMI-1640 (GE Healthcare Life Sciences) containing 10% FBS (v/v). Cells were maintained at 37 °C with 5% CO₂. The cell lines were subcultured by enzymatic digestion with 0.25% trypsin/1 mM EDTA solution (GE Healthcare Life Sciences, Chicago, IL, USA) when they were 80% confluent. All cell lines tested negative for Mycoplasma contamination.