13c zen

LCMS-grade acetonitrile, methanol, ammonia acetate, formic acid, ammonia water, ammonium hydrogen carbonate and ammonium dihydrogen phosphate were commercially obtained from Fisher Scientific (USA). Mycotoxin standards for AFB₁, AFB₂, AFG₁, AFG₂, AFM₁, AFM₂, HT2, T2, 3A-DON, 15A-DON, FB₁, FB₂, FB₃, OTA, OTB, AOH, AME, TeA, BEA, TEN, ALT, MON, DON, NIV, Fus X, ZEN, ZAN, α-ZOL, β-ZOL, α-ZAL, β-ZAL, PAT, DON-3-G, DOM-1, NEO, DAS, SMC, CIT, and CPA were obtained from Biopure (Austria). ENNs (ENNA₁, ENNA, ENNB₁, and ENNB) were supplied by PriboLab (Singapore). A total of 21 labeled internal standards were utilized, with ¹³C-DON, ¹³C-3-ADON, ¹³C-NIV, ¹³C-AFB₁, ¹³C-AFB₂, ¹³C-AFG₁, ¹³C-AFG₂, ¹³C-AFM₁, ¹³C-ZEN, ¹³C-T2, ¹³C-HT2, ¹³C-PAT, ¹³C-DAS, ¹³C-SMC, ¹³C-FB₁, ¹³C-FB₂, ¹³C-FB₃, ¹³C-OTA, and ¹³C-CIT provided by Biopure, ¹³C-TeA supplied by Fluka (USA), and TEN-d₃ obtained from TRC (Canada). Ultrapure water was obtained on a Milli-Q system (Millipore, USA). The MycoSep 226 Aflazon+ multifunctional cartridge and MultiSep 211 Fum column were supplied by Romer Labs (Austria). The Oasis HLB SPE column (200 mg, 6 mL) was purchased from Waters (USA).