Rab11ep3017

Manufactured by Bloomington Drosophila Stock Center

The Rab11EP3017 is a genetic tool used in Drosophila research. It functions as a reporter construct that allows for the visualization and analysis of the expression pattern of the Rab11 gene, which is involved in intracellular vesicle trafficking.

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2 protocols using rab11ep3017

Drosophila Endocytic Trafficking Regulators

Cited 2 times

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All crosses and staging were performed at 25°C. Stocks are described in FlyBase (http://flybase.bio.indiana.edu). UAS-YFP-Rab11, UAS-YFP-Rab11^Q70L, UAS-YFP-Rab11^S25N, UAS-Rab11-RNAi, UAS-Cdc42^V12, UAS-YFP-Rab8^Q67L, UAS-YFP-Rab4, UAS-YFP-Rab14^Q97L, UAS-aPKC-RNAi, UAS-Rho1, Dia⁵ FRT40A, UAS-YFP-Rab5, GMR-gal4, Nuf^EY11342, Rho1^72F, Cdc42⁴ FRT19A, UAS-Sec8, Rab11^EP3017, UAS-Rok-CAT, UAS-LIMK-HA, UAS-MBS-RNAi, and Sqh^AX3; RLC-GFP were provided by the Bloomington Stock Center (Indiana University, Bloomington, IN); UAS-Rab5-RNAi was provided by the Vienna Drosophila RNAi Center (Vienna, Austria); UAS-Rok-RNAi and UAS-PKN-RNAi were provided by the National Institute of Genetics (Mishima, Japan); Shg^R69 FRT42D was provided by C. Micchelli (Washington University, St. Louis, MO); Par6^Δ226 FRT19A and UAS-aPKC^CAAX were provided by C. Doe (University of Oregon, Eugene, OR); Sec5^E10 FRT40A and UAS-Sec5 were provided by T. L. Schwarz (Harvard Medical School, Boston, MA); dRip11^d00190 was provided by the Exelixis Collection (Harvard Medical School); and UAS-Rho1 RNAi, UAS-Cdc42 RNAi, and UAS-Cdc42 were generated by our lab (Warner and Longmore, 2009a (link), b (link)).

Yashiro H., Loza A.J., Skeath J.B, & Longmore G.D. (2014). Rho1 regulates adherens junction remodeling by promoting recycling endosome formation through activation of myosin II. Molecular Biology of the Cell, 25(19), 2956-2969.

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Drosophila Membrane Transport Mechanisms

Cited 1 time

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Experiments utilized w¹¹¹⁸ as the wild-type control strain. UAS-Serp-CBD-GFP line [27 (link)] is from Dr. Stephen Lustig. btl-gal4 line [86 (link)] is from Dr. Mark Krasnow. DE-cad:GFP line [65 (link)] is from Dr. Yang Hong. UAS-rab11 [87 (link)] and UAS-rab7DN [87 (link)] lines are from Dr. Marcos González-Gaitán. UAS-rab4-YFP, UAS-rab7DN-YFP and UAS-rab11-GFP [58 (link)], UAS-Lamp-GFP [59 (link)], Rab11^[EP3017) [67 (link)], FRT82B rab7Gal4-knock-in/TM3 [68 (link)], Drop/TM3GFP [88 (link)], and P{nos-Cas9.R}attP40 [89 (link)], are from Bloomington stock center. P{TKO.GS01947}attP40 and P{TKO.GS01948}attP40 gRNA lines are from DRSC/TRiP functional genomics resources & DRSC-BTRR (

https://fgr.hms.harvard.edu/fly-in-vivo-rnai

Scholl A., Ndoja I., Dhakal N., Morante D., Ivan A., Newman D., Mossington T., Clemans C., Surapaneni S., Powers M, & Jiang L. (2023). The Osiris family genes function as novel regulators of the tube maturation process in the Drosophila trachea. PLOS Genetics, 19(1), e1010571.

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