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Phsl ser563

Manufactured by Santa Cruz Biotechnology
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The PHSL Ser563 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is used for the detection and quantification of specific proteins or molecules in biological samples. The core function of this product is to facilitate analytical procedures, but no further details can be provided without making assumptions about its intended use.

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Lab products found in correlation

Anti flag m2 antibody, by Merck Group (2 mentions) Chemidoc mp imaging station, by Bio-Rad (2 mentions) Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions) Western lightning, by PerkinElmer (2 mentions) P perilipin, by Merck Group (2 mentions) Phsl ser660, by Cell Signaling Technology (2 mentions) Fluorophore conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Erk1 2, by Santa Cruz Biotechnology (2 mentions) P erk1 2, by Santa Cruz Biotechnology (2 mentions) Pvdf membrane, by Merck Group (2 mentions)

2 protocols using phsl ser563

1

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein samples were denatured in Laemmli buffer, and loaded onto SDS-PAGE gels and transferred to PVDF membranes (Millipore, Billerica, MD). Membranes were then incubated overnight at 4 °C in wash buffer (10 mM Tris, pH 7.5, 100 mM NaCl, 0.1% Tween-20) containing 1% BSA with the primary antibody. Antibodies were obtained from Cell Signaling Technology [pHSL Ser660 (Cat. 4126), pHSL Ser563 (Cat. 4139), HSL (Cat. #4107), pERK1/2 (Cat. 4370), and ERK 1/2 (Cat. 4695)], Santa Cruz Biotechnology [Actin (Cat. #SC69879)], Vala Biosciences [p-perilipin (Cat. 4854), perilipin (Cat. 4856)], and Sigma [M2 anti-FLAG antibody (Cat. F1804)]. The membrane was then rinsed with wash buffer and incubated for 1 h at room temperature in wash buffer containing 1% BSA with 1:2500 HRP-conjugated secondary antibody (Santa Cruz Biotechnology) or 1:2500 fluorophore-conjugated secondary antibody (Life Technologies). After further washing, membranes were visualized using enhanced chemiluminescence (Western Lightning, Perkin-Elmer) or imaged directly for fluorescent secondary antibodies and recorded on a BioRad ChemiDoc MP imaging station.
Krishnamoorthy L., Cotruvo JA J.r., Chan J., Kaluarachchi H., Muchenditsi A., Pendyala V.S., Jia S., Aron A.T., Ackerman C.M., Vander Wal M.N., Guan T., Smaga L.P., Farhi S.L., New E.J., Lutsenko S, & Chang C.J. (2016). Copper Regulates Cyclic AMP-Dependent Lipolysis. Nature chemical biology, 12(8), 586-592.
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2

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein samples were denatured in Laemmli buffer, and loaded onto SDS-PAGE gels and transferred to PVDF membranes (Millipore, Billerica, MD). Membranes were then incubated overnight at 4 °C in wash buffer (10 mM Tris, pH 7.5, 100 mM NaCl, 0.1% Tween-20) containing 1% BSA with the primary antibody. Antibodies were obtained from Cell Signaling Technology [pHSL Ser660 (Cat. 4126), pHSL Ser563 (Cat. 4139), HSL (Cat. #4107), pERK1/2 (Cat. 4370), and ERK 1/2 (Cat. 4695)], Santa Cruz Biotechnology [Actin (Cat. #SC69879)], Vala Biosciences [p-perilipin (Cat. 4854), perilipin (Cat. 4856)], and Sigma [M2 anti-FLAG antibody (Cat. F1804)]. The membrane was then rinsed with wash buffer and incubated for 1 h at room temperature in wash buffer containing 1% BSA with 1:2500 HRP-conjugated secondary antibody (Santa Cruz Biotechnology) or 1:2500 fluorophore-conjugated secondary antibody (Life Technologies). After further washing, membranes were visualized using enhanced chemiluminescence (Western Lightning, Perkin-Elmer) or imaged directly for fluorescent secondary antibodies and recorded on a BioRad ChemiDoc MP imaging station.
Krishnamoorthy L., Cotruvo JA J.r., Chan J., Kaluarachchi H., Muchenditsi A., Pendyala V.S., Jia S., Aron A.T., Ackerman C.M., Vander Wal M.N., Guan T., Smaga L.P., Farhi S.L., New E.J., Lutsenko S, & Chang C.J. (2016). Copper Regulates Cyclic AMP-Dependent Lipolysis. Nature chemical biology, 12(8), 586-592.
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