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Mitotracker deep red m22426

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MitoTracker Deep Red (M22426) is a fluorescent stain used to label mitochondria in live cells. It is a cell-permeant dye that accumulates in active mitochondria, emitting a deep red fluorescence. The dye can be used to visualize and study mitochondrial morphology and dynamics in a variety of cell types.

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Lab products found in correlation

Anti vdac1, by Abcam (1 mentions) Anti parkin, by Santa Cruz Biotechnology (1 mentions) Anti tom20, by Santa Cruz Biotechnology (1 mentions) Anti beclin1, by Abcam (1 mentions) Anti parkin, by Abcam (1 mentions) Anti mitofusin 2, by Cell Signaling Technology (1 mentions) Anti pacs 2, by Proteintech (1 mentions) Anti kdel, by Enzo Life Sciences (1 mentions) Anti grp75, by Cell Signaling Technology (1 mentions) Anti myc, by Cell Signaling Technology (1 mentions) Glucose g7021, by Merck Group (1 mentions) Anti lc3b, by Cell Signaling Technology (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Mitosox red m36008, by Thermo Fisher Scientific (1 mentions) Ab131607, by Abcam (1 mentions) Ab50838, by Abcam (1 mentions) Ab157457, by Abcam (1 mentions) Ab56783, by Abcam (1 mentions) Ab106814, by Abcam (1 mentions) Gapdh 5174, by Cell Signaling Technology (1 mentions)

4 protocols using mitotracker deep red m22426

1

Mitochondria-Endoplasmic Reticulum Interactions

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Oligomycin (O4876), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) (C2920), antimycin A (A8674), rotenone (R8875), carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (C2759), bafilomycin A1 (B1793), sodium pyruvate (P5280), and glucose (G7021) were purchased from Sigma-Aldrich (St. Quentin Fallavier, France). MitoTracker Deep Red (M22426) and l-glutamine (25030-024) were purchased from ThermoFisher Scientific (Illkirch, France). Cyto-ID (ENZ-51031) was purchased from Enzo Life Sciences (Villeurbanne, France). Anti-Mitofusin 2 (rabbit, 9482), anti-Grp75 (rabbit, 3593), anti-LC3B (rabbit, 2775), and anti-Myc (mouse, 2276) were from Cell Signaling Technology (Saint Quentin Yvelines, France). Anti-Tom20 (rabbit, sc-11415), Anti-Tom20-AF488 (rabbit, sc-17764), anti-Parkin (mouse, sc-32282), and anti-Ero1-Lα (mouse, sc-365526) were from Santa Cruz Biotechnology (Heidelberg, Germany). Anti-VDAC1 (mouse, ab14734), anti-Parkin (rabbit, ab15954), and anti-Beclin-1 (mouse, ab114071) were from Abcam (Paris, France). Anti-KDEL (mouse, ADI-SPA-827-D) was from Enzo Life Sciences. Anti-PACS-2 (rabbit, 19508-1-AP) was from Proteintech (Manchester, UK), and Anti-PACS-2 (rabbit, clone 18143) was previously reported [9 (link)]. Anti-IP3R1 (rabbit, 07-1213) was from Merck Millipore (Molsheim, France). Anti-β-actin (mouse, A2228) and anti-P62 (rabbit, P0067) were from Sigma-Aldrich.
Moulis M., Grousset E., Faccini J., Richetin K., Thomas G, & Vindis C. (2019). The Multifunctional Sorting Protein PACS-2 Controls Mitophagosome Formation in Human Vascular Smooth Muscle Cells through Mitochondria-ER Contact Sites. Cells, 8(6), 638.
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2

Multiparametric flow cytometry analysis

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The cells (5×10 5 ) cells were stained with the following antibodies purchased from BD Bioscience (Le Pont-De-Claix, France): phycoerythrin (PE)-labeled anti-mouse CD86 (clone GLI, 553692), Peridinin-chlorophyll proteins (PerCp)-Cyanin 5.5-labeled anti-mouse CD19 (clone ID3, 551001), PE-labelled anti-mouse CD62L (clone MEL-14, 553151), allophycocyanin (APC)-labeled anti-mouse CD44 (clone IM7, 559250) and fluorescein isothyocyanate (FITC)-labeled annexin-V (560931). The fixable viability dye (FVD) efluor 780 (65-0865-14), the mitochondrial superoxide indicator MitoSOX™ Red (M36008), MitoTracker™ Green FM (M7514) and MitoTracker Deep Red (M22426) were purchased from Thermo Fisher Scientific (Fisher Scientific, Pittsburgh, PA, USA,).
Murera D ., Malaganahalli S ., Martín C ., Reina G ., Fauny JD ., Dumortier H ., Vázquez E , & Bianco A . (2019). Few layer graphene does not affect the function and the autophagic activity of primary lymphocytes. Nanoscale, 11(21).
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3

Sperm Viability Assessment via Fluorescence Probes

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All chemicals and reagents used for this experiment were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The powdered egg yolk was obtained from NIVE (Nunspeet Holland Eiproducten, Nunspeet, The Netherlands). The fluorescence probes and analysis kits ((LIVE/DEAD® sperm viability kit (L-7011; PI and SYBR-14) and Mitotracker deep red (M22426)) were all purchased from Invitrogen SA (Barcelona, Spain). All fluorochromes were stored at −20 °C in the dark. The washing media used for single layer centrifugation (SLC) was Bovipure® (Nidacon, Mölndal, Sweden) while the control incubation media used was a modified phosphate buffer solution (PBS solution supplemented with 36 μg/mL pyruvate and 0.5 mg/mL BSA with an osmolarity of 280–300 mOsm/kg and a pH of 7.3–7.4) and an in-vitro fertilization commercial media (BO-IVF, IVF Biosciences, Falmouth, UK).
Ohaneje U.L., Osuagwuh U.I., Alvarez-Rodríguez M., Yánez-Ortiz I., Tabarez A, & Palomo M.J. (2021). The Re-Addition of Seminal Plasma after Thawing Does Not Improve Buck Sperm Quality Parameters. Animals : an Open Access Journal from MDPI, 11(12), 3452.
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4

Mitochondrial Function Regulation Protocol

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Cell Counting Kit-8 (CCk8; CK04) was purchased from Dojindo Laboratories (Kumamoto, Japan). Glass-bottom cell culture dishes (801001) were purchased from Nest Biotechnology (Wuxi, China). DM (mast-120131108, HPLC ≥98%) was purchased from MUST Biotechnology Co., Ltd. (Chengdu, China). Dex (D4902), Dex (D1756), Akti (A6730), rapamycin (RAPA; R0395) and the Protein Carbonyl Content Assay Kit (MAK094) were purchased from Sigma-Aldrich (St. Louis, MO, USA). MitoTracker Deep Red (M22426) and MitoSOX Red (M36008) were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against myosin heavy chain (MHC; ab 24642), PGC-1α (ab 106814), TFAM (ab 131607), OPA1 (ab 157457), Tom20 (ab 56783), mfn1 (ab 104585), mfn2 (ab 50838), FoxO3a (ab 23683) and VDAC1 (ab 15895) were obtained from Abcam (Cambridge, UK). Antibodies against p-Akt (9271), Akt (9272), p-mTOR (2971), mTOR (2972), p-S6K (9205), S6K (9202) and GAPDH (5174) were obtained from Cell Signaling Technology (Beverly, MA). Antibodies against atrogin-1 (sc-33782) and MuRF1 (sc-27642) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), whereas the antibody against drp1 (BD611113) was obtained from BD Biosciences (San Jose, CA).
Huang Y., Chen K., Ren Q., Yi L., Zhu J., Zhang Q, & Mi M. (2018). Dihydromyricetin Attenuates Dexamethasone-Induced Muscle Atrophy by Improving Mitochondrial Function via the PGC-1α Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(2).
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