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Goat anti glun1

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Goat anti-GluN1 is a primary antibody that recognizes the GluN1 subunit of the N-methyl-D-aspartate (NMDA) receptor. It is produced in goats and can be used for the detection and quantification of the GluN1 protein in various applications such as Western blotting, immunohistochemistry, and immunoprecipitation.

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Lab products found in correlation

Rabbit anti camk 2, by Santa Cruz Biotechnology (2 mentions) Immobilon western, by Merck Group (2 mentions) Rabbit anti glua2 c terminal, by Synaptic Systems (2 mentions) Rabbit anti gad65 67, by Merck Group (2 mentions) Rabbit anti gapdh antibody, by Merck Group (2 mentions) Rabbit anti homer1, by Synaptic Systems (2 mentions) Mouse anti synapsin1a b, by Santa Cruz Biotechnology (2 mentions) Fusion fx7 system, by Vilber (2 mentions) Mouse anti gephyrin, by Synaptic Systems (2 mentions) Rabbit anti arc, by Synaptic Systems (2 mentions) Rabbit anti vglut1, by Synaptic Systems (2 mentions) Hrp conjugated secondary antibody, by GE Healthcare (1 mentions)

2 protocols using goat anti glun1

1

Immunoblotting Protocol for Synaptic Proteins

Cited 1 time
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Protein extracts were resolved by SDS-PAGE, transferred onto nitrocellulose membrane (BioTraceNT, PALL), immunoblotted with the following primary antibodies: mouse anti-FMRP18 (link) 1 µg/mL (DSHB, Clone 2F5-1); Rabbit anti-GluA1 C-terminal 1/1000 (Merk-Millipore #AB1504); Rabbit anti-GluA2 C-terminal 1/2000 (Synaptic System #182103); Rabbit anti-Homer1 1/1000 (Synaptic Systems #160003); Mouse anti-PSD95 1/10000 (NeuroMab #75-028 clone K28/43); Goat anti-GluN1 1/500 (Santa-Cruz #sc-1467); Mouse anti-Synapsin1a/b 1/500 (Santa-Cruz #sc-376623); Rabbit anti-GAD65/67 1/500 (Merk-Millipore #ABN904); Rabbit anti-CaMKII 1/500 (Santa-Cruz #sc-9035); Rabbit anti-Arc 1/1000 (Synaptic Systems #156003); Mouse anti-Gephyrin 1/1000 (Synaptic System #147111); Rabbit anti-vGluT1 1/5000 (Synaptic System #135303). Standard loading controls were included using a rabbit anti-GAPDH antibody 1/25000 (Sigma #G9545) or a rabbit anti-β3 Tubulin 1/25000 (Synaptic Systems #302302) as indicated. Proteins were revealed using the appropriate HRP-conjugated secondary antibodies (GE healthcare #NA931V and #NA934V). Proteins were then identified using Immobilon Western (Millipore) chemiluminescent solution and images acquired on a Fusion FX7 system (Vilber Lourmat). Full-size blots for cropped gels are shown in the Source data file.
Prieto M., Folci A., Poupon G., Schiavi S., Buzzelli V., Pronot M., François U., Pousinha P., Lattuada N., Abelanet S., Castagnola S., Chafai M., Khayachi A., Gwizdek C., Brau F., Deval E., Francolini M., Bardoni B., Humeau Y., Trezza V, & Martin S. (2021). Missense mutation of Fmr1 results in impaired AMPAR-mediated plasticity and socio-cognitive deficits in mice. Nature Communications, 12, 1557.
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2

Western Blotting of Synaptic Proteins

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Protein extracts were resolved by SDS-PAGE, transferred onto nitrocellulose membrane (BioTraceNT, PALL), immunoblotted with the following primary antibodies: mouse anti-FMRP 18 ; Rabbit anti-GluA1 C-terminal 1/1000 (Merk-Millipore); Rabbit anti-GluA2 C-terminal 1/2000 (Synaptic System); Rabbit anti-Homer1 1/1000 (Synaptic Systems); Mouse anti-PSD95 1/10000 (NeuroMab); Goat anti-GluN1 1/500 (Santa-Cruz); Mouse anti-Synapsin1a/b 1/500 (Santa-Cruz); Rabbit anti-GAD65/67 1/500 (Merk-Millipore); Rabbit anti-CaMKII 1/500 (Santa-Cruz); Rabbit anti-Arc 1/1000 (Synaptic Systems); Mouse anti-Gephyrin 1/1000 (Synaptic System); Rabbit anti-vGluT1 1/5000 (Synaptic System). Standard loading controls were included using a rabbit anti-GAPDH antibody 1/25000 (Sigma) or a rabbit anti-b3 Tubulin 1/25000 (Synaptic Systems) as indicated.
Proteins were revealed using the appropriate HRP-conjugated secondary antibodies (GE healthcare). Proteins were then identified using Immobilon Western (Millipore) chemiluminescent solution and images acquired on a Fusion FX7 system (Vilber Lourmat). Full-size blots for cropped gels are shown in Supplementary figure 7.
Prieto M., Folci A., Poupon G., Schiavi S., Buzzelli V., François U., Pousinha P., Lattuada N., Abélanet S., Pronot M., Castagnola S., Chafaï M., Khayachi A., Brau F., Deval E., Francolini M., Bardoni B., Humeau Y., Trezza V., & Martin S. (2020). Abnormal AMPAR-mediated synaptic plasticity, cognitive and autistic-like behaviors in a missense Fmr1 mutant mouse model of Fragile X syndrome.
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