Normal goat igg control ab 108 c

Patient samples were obtained after informed consent in accordance with the Declaration of Helsinki in the hematology departments involved in this study. Mononuclear cells (MNCs) from 54 blood and BM samples were obtained from AML, excluding acute promyelocytic leukemia, patients at diagnosis. AML characteristics are presented in Table S1. Normal samples correspond to steady-state peripheral blood and BM samples from healthy donors for allogeneic BM transplantation, collected after informed consent. When necessary, primary cells were maintained in IMDM culture medium containing 10% fetal calf serum (FCS). KG1A myeloid leukemia cells were cultured in RPMI-1640 medium containing 10% FCS. BMP4 and LDN-193189 (20 nM) (Sigma-Aldrich) were added in serum-free medium as indicated^{18 (link),20 (link)}. Normal goat IgG control (AB-108-C) and anti-hBMPR1A (AF346) (R&D Systems) were used at 4 µg/mL.

The antibodies used were rabbit anti-p44/42 MAPK (Erk1/2) antibody #9102 (Cell Signaling Technology, Beverly, MA), rabbit anti-phospho-Erk1/2 antibody #9101 (Cell Signaling Technology), rabbit anti-phospho-p38 MAPK antibody #4511 (Cell Signaling Technology), rabbit anti-p38 MAPK antibody #8690 (Cell Signaling Technology), rabbit anti-phospho-SAPK/JNK antibody #4668 (Cell Signaling Technology), rabbit anti-SAPK/JNK antibody #9258 (Cell Signaling Technology), mouse anti-phospho-IκBα #9246 (Cell Signaling Technology), mouse anti-IκBα antibody #4814 (Cell Signaling Technology), rabbit anti-LTβR, N-Terminal antibody #SAB4501788 (Sigma-Aldrich), goat anti-LTβR antibody #L5412 (Sigma-Aldrich), normal goat IgG control #AB-108-C (R&D systems), anti-mouse-IgG HRP-linked antibody #7076 (Cell Signaling Technology), and anti-rabbit IgG-HRP-linked antibody #7074 (Cell Signaling Technology).
Equal protein loading was confirmed by probing the blot with mouse anti-α-tubulin (Sigma-Aldrich) antibody.