Flexigen dna kit

Using 30 ml of blood from Streck tubes, plasma was separated from the cellular fraction by centrifugation at 1600g and then at 14,000g for 10 min/each. Plasma samples were frozen at − 80 °C until further processing. Cell-free DNA (cf-DNA) was extracted from 4 ml of thawed plasma samples using the QIAsymphony DSP Circulating DNA Kit (Qiagen, Courtaboeuf, France) and the QIASymphony instrument, according to the manufacturer’s protocol. Concentrated and purified cf-DNA was eluted in a final volume of 60 μl of AVE buffer.
Parental genomic DNA was extracted from whole blood collected in EDTA tubes with the FlexiGen DNA kit (Qiagen) according to the manufacturer’s protocol for genotyping.

DNA samples of all participants were extracted using the Flexigen DNA kit (QIAGEN). The quantity and the quality of the extracted DNA were measured using the DENOVIX DS-11 nanodrop spectrophotometer. Then, WES was performed at Macrogen (South Korea). Briefly, exome capture was carried out using Agilent SureSelect V6, and massive parallel sequencing was then performed on an Illumina Novaseq 6000 system to generate 151-bp paired-end reads.

Genomic DNA was isolated from 5 mL of peripheral blood with the flexiGen DNA kit from Qiagen according to manufactured protocol and then diluted to a final concentration of 200 ng/μL.