Aa800 spectrometer

Manufactured by Agilent Technologies

The AA800 spectrometer is a versatile analytical instrument designed for atomic absorption spectroscopy. It provides accurate and reliable measurements of elemental concentrations in various sample types.

Automatically generated - may contain errors

Lab products found in correlation

0.1 m hcl, by Merck Group (1 mentions) Suprapur hno3, by Merck Group (1 mentions)

2 protocols using aa800 spectrometer

Quantifying SPION Trace Elements

Check if the same lab product or an alternative is used in the 5 most similar protocols

The iron content in the SPIONs was measured using an AAS instrument (Varian AA800 spectrometer, with acetylene/air flame atomization). An iron standard solution (1,000 mg/L in HNO₃; Merck, Darmstadt, Germany) was used for the calibration curve. Samples of dried SPIONs were digested in nitric acid (trace analysis grade, EM-1.00441.1000, 65% [w/v]; Millipore, Billerica, MA, USA), followed by a dilution with 0.1 M HCl (Merck) prior to the measurement.
Concentrations of cobalt, gallium, and copper were analyzed using a GF-AAS (Agilent 240FS AA spectrometer with a Zee-man graphite tube atomizer). The standard solutions of Co(II), Ga(III), and Cu(II) at 25, 50, and 40 ppb, respectively, in 0.1 M HCl were used for the calibration curve of each element.

Pham T.N., Lengkeek N.A., Greguric I., Kim B.J., Pellegrini P.A., Bickley S.A., Tanudji M.R., Jones S.K., Hawkett B.S, & Pham B.T. (2017). Tunable and noncytotoxic PET/SPECT-MRI multimodality imaging probes using colloidally stable ligand-free superparamagnetic iron oxide nanoparticles. International Journal of Nanomedicine, 12, 899-909.

+ Open protocol

+ Expand

Quantification of Iron Content in s-SPIONs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The iron content in the s-SPIONs was measured using an AAS instrument (Varian AA800 spectrometer, with acetylene/air flame atomization). An iron standard solution (1000 mg/L in HNO₃, Merck) was used to prepare 2, 4, 6, 8, and 100 ppm standard solutions for the calibration curve. Samples of dried s-SPIONs were digested in Suprapur^® HNO₃ (500 µL for each sample, Merck-Millipore) at 70 °C, followed by dilution with 0.1 M HCl (Merck) to a final volume of 5.0 mL prior to measurement.
Either graphite furnace atomic absorption spectroscopy (GF-AAS, Agilent 240FS AA spectrometer with a Zeeman graphite tube atomizer; Agilent Technologies Australia, Mulgrave, VIC, Australia) or flame-AAS (Varian AA800 spectrometer, with acetylene/air flame atomization; Agilent Technologies Australia) were used to quantify iron content in the collected frozen tissues. For GF-AAS, a standard solution of iron in 1% (v/v) HNO₃ at a concentration of 25 ppb was prepared from a commercial iron standard (Merck Millipore, 1000 mg/L in HNO₃,) for a calibration curve at 5, 10, 15, and 20 ppb.

Pham B.T., Colvin E.K., Pham N.T., Kim B.J., Fuller E.S., Moon E.A., Barbey R., Yuen S., Rickman B.H., Bryce N.S., Bickley S., Tanudji M., Jones S.K., Howell V.M, & Hawkett B.S. (2018). Biodistribution and Clearance of Stable Superparamagnetic Maghemite Iron Oxide Nanoparticles in Mice Following Intraperitoneal Administration. International Journal of Molecular Sciences, 19(1), 205.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!