Cleaved caspase 3 specific antibody

Manufactured by Cell Signaling Technology

The Cleaved caspase-3-specific antibody is a laboratory reagent used to detect the presence of the cleaved form of the caspase-3 protein. Caspase-3 is a key enzyme involved in the apoptosis (programmed cell death) pathway. The antibody specifically recognizes the cleaved, activated form of caspase-3, which is a marker of cells undergoing apoptosis.

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Lab products found in correlation

Fluorescein isothiocyanate conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Bz x700 fluorescence microscope, by Keyence (1 mentions)

Close spelling variants of this product

Primary antibody specific for cleaved caspase-3 Cleaved caspase-3 antibody Cleaved caspase-3 Caspase-3 antibody Caspase-3 Caspases

2 protocols using cleaved caspase 3 specific antibody

Azide-Induced Apoptosis in A549 Cells

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A549 cells were plated on glass coverslips and then treated with indicated amounts of azide in the presence or absence of 25 μM cobinamide for 24 hours. The cells were fixed in 3.7% paraformaldehyde, permeabilized with 1% Triton-X-100, and incubated with a cleaved caspase-3-specific antibody (1:100 dilution) (Cell Signaling) overnight at 4°C. After incubation with a fluorescein isothiocyanate-conjugated secondary antibody (1:100 dilution) (Jackson ImmunoResearch, West Grove, PA), nuclei were stained using Hoechst 33342 dye. Immunofluorescence images were recorded using a Keyence BZ-X700 fluorescence microscope.

Ruiz-Avila L., Burgess S.R., Stiefel V., Ludevid M.D, & Puigdomènech P. (1992). Accumulation of cell wall hydroxyproline-rich glycoprotein mRNA is an early event in maize embryo cell differentiation.. Proceedings of the National Academy of Sciences of the United States of America, 89(6), 2414-2418.

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Apoptosis Quantification in Mouse Cecum

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Mouse cecal tissue was fixed in Bouin’s solution for 24 h and washed with 70% ethanol. Paraffin-embedded cecal sections were stained at the biorepository core facility of the University of Virginia using cleaved caspase-3-specific antibody (catalog no. 9661L; Cell Signaling). The numbers of caspase-3-positive (brown) epithelial cells and crypts were scored by investigators blinded to mouse treatments. Data were analyzed using Mann-Whitney test.

Wojcik G.L., Marie C., Abhyankar M.M., Yoshida N., Watanabe K., Mentzer A.J., Carstensen T., Mychaleckyj J., Kirkpatrick B.D., Rich S.S., Concannon P., Haque R., Tsokos G.C., Petri WA J.r, & Duggal P. (2018). Genome-Wide Association Study Reveals Genetic Link between Diarrhea-Associated Entamoeba histolytica Infection and Inflammatory Bowel Disease. mBio, 9(5), e01668-18.

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