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Horseradish peroxidase conjugated goat anti rabbit secondary antibodies

Manufactured by ABclonal
Sourced in United States

Horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies are a type of laboratory reagent used for detection and quantification of target proteins in various immunoassays, such as Western blotting and enzyme-linked immunosorbent assay (ELISA). These antibodies are produced by immunizing goats with rabbit immunoglobulins and conjugating the resulting antibodies with the enzyme horseradish peroxidase.

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2 protocols using horseradish peroxidase conjugated goat anti rabbit secondary antibodies

1

Osteoclastogenesis Pathway Molecular Assays

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The 12 ODNs, 2006, β-actin, Nfatc, c-fos, RANK, MMP9, and FAM labeled ODNs were synthesized by Takara (Dalian, China), and the sequences are listed in ESI Table 1. The PrimerScript® RT reagent kit and SYBR Green Premix Ex Taq kit were purchased from Takara. The antibodies (cyclin A2, cyclin B1, cyclin D1, cyclin E1, Nfatc, c-fos, RANK, MMP9, OPG, RANKL, β-actin, and horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies) were all purchased from ABclonal (Boston, USA).The RAW264.7 cells (SCSP-5036) were purchased from Cell library of Chinese Academy of Sciences.
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2

Western Blot Analysis of Cellular Proteins

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Total protein of the cells was isolated with RIPA tissue lysis Buffer (Solarbio, Beijing, China) in accordance with the operating manual. The concentration of the obtained protein was measured with BCA. Equal amounts (50 mg) of protein were separated on a 10% sodium dodecyl sulfate polyacrylamide gel and transferred to polyvinylidene fluoride membranes (Thermo Scientific). Membranes were incubated with primary rabbit antibodies against ANLN antibody (1 : 3000, 66643-1; Proteintech), RhoA (1 : 1000, 2117; Cell Signaling Technology, Danvers, MA, USA), and β-actin (1 : 5000, AC026; ABclonal) at 37°C overnight. The membranes were incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibodies (1 : 10000; ABclonal) for 1 h at room temperature. The signals were detected with an enhanced chemiluminescence detection reagent (Thermo Scientific). The bands were quantified via densitometry and analyzed with Quantity One software (Bio-Rad Laboratories, Hercules, CA, USA). Relative protein levels were normalized to β-actin.
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