HK2 cells, deriving from human renal proximal tubule epithelial cell line, were cultured in an extracellular matrix (ECM) medium (Gibco Laboratories, Gaithersburg, MD, USA) with fetal bovine serum at concentration of 10% contained (Gibco Laboratories).
Ecm medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
ECM medium is a cell culture media product designed for the growth and maintenance of extracellular matrix-producing cells. It provides the necessary nutrients and growth factors to support the cultivation of these specialized cell types.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Huvecs, by Thermo Fisher Scientific (2 mentions)
Human umbilical vein endothelial cells (huvec), by Thermo Fisher Scientific (1 mentions)
Hmec 1, by Thermo Fisher Scientific (1 mentions)
Mc3t3 e1, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Huvecs, by American Type Culture Collection (1 mentions)
Propranolol, by Merck Group (1 mentions)
β elemene, by Merck Group (1 mentions)
Hemecs, by Thermo Fisher Scientific (1 mentions)
L 15 medium, by Thermo Fisher Scientific (1 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (1 mentions)
Huvecs, by ScienCell (1 mentions)
5 protocols using ecm medium
1
HK2 Cells Culture in ECM Medium
2
Cell Culture Conditions Optimized
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Cell lines HUVEC, HMEC‐1 and MC3T3‐E1 were purchased from CAS (Chinese Academy of Sciences, Shanghai, China). HUVEC and MC3T3‐E1 cells were cultured in DMEM medium (Gibco, Gaithersburg, MD, USA). HMEC‐1 cells were cultured in ECM medium (Gibco). Medium contained 10% foetal bovine serum (FBS), and all cells were cultured in a humidified atmosphere consisting of 5% CO2 and 95% air at 37°C.
3
Endothelial Cell Responses to β-elemene and Propranolol
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HemECs were purchased from the Institute of Otwo Biotech (Shenzhen, China), and human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). Cells were cultured at 37 °C under 5% CO2 and 95% air, HemECs were maintained in a DMEM medium supplemented with 10% FBS (Gibco, Billings, MT, USA) and 1% penicillin-streptomycin (FBS, Hyclone, Logan, UT, USA), HUVECs were maintained in ECM medium (Gibco, Billings, MT, USA). HemECs were exposed to either β-elemene (Dalian Holley Kingkong Pharmaceutical Co., Dalian, China) or propranolol (Sigma-Aldrich, Merck, St. Louis, MO, USA), or a combined treatment of β-elemene and propranolol for 24 h prior to analysis.
4
Investigating Paracrine Effects of hUC-MSCs on Inflammatory Response in HUVECs
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We firstly collected the CM of hUC-MSC as follows: cells were cultured in complete medium up to 90% confluency, the cells were washed with PBS and changed with α-MEM basic medium (Life Technology) for another 24 h, and the medium was collected as hUC-MSC-CM. Then HUVECs were cultured to 90% confluency in ECM complete medium containing ECM medium (Life Technology) with 10% FBS and then changed with mixed medium which contained 50% ECM complete medium and 50% hUC-MSC-CM. ECM medium supplemented with 5% FBS was used as a control medium. Ten nanograms/milliliter TNF-α was used to stimulate HUVECs for 24 h. The HUVECs and cell supernatant were then collected to assess the expression of TNF-α, IL-1β, p-P65, and p-P38 and Selectin-E by real-time PCR and ELISA.
5
Cell Culture Conditions for SW579, TPC-1, and HUVEC
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SW579 and TPC-1 cells were purchased from the ATCC. HUVECs were purchased from ScienCell (USA). SW579 cells were incubated in L-15 medium (GIBCO, Cat. no. 41300039), and TPC-1 cells grown in Minimum essential medium (MEM; GIBCO, Cat. no. 41500034). HUVECs were cultured in ECM medium (Life Technologies). The cultures all contained 10% fetal bovine serum (Gibco, USA). And both types of cells were all incubated in an incubator at 37 °C with 5% CO2.
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